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. 1992 May 11;20(9):2361–2366. doi: 10.1093/nar/20.9.2361

Identification of exon sequences and an exon binding protein involved in alternative RNA splicing of calcitonin/CGRP.

G J Cote 1, D T Stolow 1, S Peleg 1, S M Berget 1, R F Gagel 1
PMCID: PMC312354  PMID: 1594453

Abstract

Transcripts derived from the 6 exon CALC I gene are differentially processed in a tissue-specific fashion to include or exclude a calcitonin-specific exon 4. All cell types which transcribe a second calcitonin/CGRP gene, CALC II, exclude exon 4. Substitution of the first 30 nucleotides of CALC I exon 4 with analogous CALC II sequence was sufficient to prevent recognition of exon 4 in in vitro or in vivo RNA splicing systems. UV crosslinking detected a approximately 66 kDa RNA-binding protein in HeLa nuclear extract which interacted with CALC I proximal exon sequence, but not CALC II or mutant sequences. UV crosslinking of this protein was inhibited by addition of nuclear extract from a cell type which normally causes exclusion of exon 4. These results identify an important regulatory element within exon 4 and support a model in which calcitonin production requires protein interaction with this sequence to facilitate exon recognition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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