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. 1992 Jul 11;20(13):3435–3441. doi: 10.1093/nar/20.13.3435

Expression of wild-type and mutant p53 proteins by recombinant vaccinia viruses.

D Ronen 1, Y Teitz 1, N Goldfinger 1, V Rotter 1
PMCID: PMC312500  PMID: 1630914

Abstract

To facilitate the purification of wild type p53 protein, we established a recombinant p53 vaccinia viral expression system. Using this efficient eukaryotic expression vector, we found that the expressed p53 proteins retained their specific structural characteristics. A comparison between wild type and mutant p53 proteins showed the conservation of the typical subcellular localization and the expression of specific antigenic determinants. Furthermore, wild type p53 exhibited a typical binding with large T antigen, whereas no binding was detected with mutant p53. Both wild type and mutant p53 proteins were highly stable and constituted 5-7% of total protein expressed in the infected cells. These expression recombinant viruses offer a simple, valuable system for the purification of wild type and mutant p53 proteins that are expressed abundantly in eukaryotic cells.

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