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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mol Nutr Food Res. 2011 Mar 4;55(7):999–1009. doi: 10.1002/mnfr.201000547

Figure 8.

Figure 8

SFN treatment selectively induces histone H3 acetylation at the P21 promoter, p21 protein and tubulin acetylation in BPH1, LnCap and PC3 but not normal PrEC prostate cells. Cells were treated with DMSO (control) (white bars) or 15 µM SFN (black bars) for the time indicated and harvested for ChIP or western blots. (A) ChIP for acetylated histone H3 at the P21 promoter at 48 h. For normalization between samples the immunoprecipitated DNA was expressed as percentage of input DNA (2CT input − CT IP × 10) and shown as fold change compared to control treatments. TSA was included as a positive control for histone acetylation (data not shown). Data were analyzed by Student’s t-test; *p<0.05. (B) Densitometry and western blots for p21 protein at 24 h. (C) Western blot and densitometry of acetylated tubulin and total tubulin at 24 and 48 h. Data in bar graphs represent mean ± SEM (n=3). Data for B and C were analyzed by one-way ANOVA; *p<0.05, **p<0.01, ***p<0.001 using Dunnett’s post-test.