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. Author manuscript; available in PMC: 2011 Jul 5.
Published in final edited form as: Epilepsia. 1999 Sep;40(9):1190–1197. doi: 10.1111/j.1528-1157.1999.tb00847.x

FIG. 3.

FIG. 3

CRH–mRNA hybridization signal in the PVN is reduced after VGB treatment. Dark-field photomicrographs of the hypothalamic paraventricular region of control (A) and VGB-treated rats (B). Animals were killed 24 h after injections of saline (A) or VGB (B). Coronal sections at the level of the PVN were subjected to in situ hybridization by using an oligodeoxynucleotide probe directed at CRH–mRNA. The sections were dipped in emulsion and developed together (see text for details). A significant reduction in CRH–mRNA signal intensity is evident after VGB treatment. (Original magnification, ×200)