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. 2011 Apr 4;21(8):1038–1048. doi: 10.1093/glycob/cwr039

Fig. 2.

Fig. 2.

Detection of Siglec-11 protein expression in human and chimpanzee adrenals and ovaries. Mouse anti-Siglec-11 antibody 4C4 was used to detect the presence of Siglec-11 (shown in red) on paraffin-embedded tissue sections. The scale bar indicates 50 μm. (A) Expression of Siglec-11 is found in macrophage-like cells inside capillaries of human adrenal cortex (upper panel). Some adrenals had infiltrating macrophages but no expression of Siglec-11 (lower panel). Mouse IgG was used as the negative reagent control. CD68 is the macrophage marker. (B) Strong expression of Siglec-11 shows on ovarian stromal cells from human and chimpanzee ovaries. Human spleen sections are used as a positive control (macrophage staining only) and mouse IgG was used as the negative reagent control. (C) Comparison of Siglec-11 expression with Vimentin (stromal marker) and CD45 (hematopoietic cell marker) in human spleen, ovary and prostate. The spleen is the positive control for Siglec-11 expression on macrophages, and the prostate sample shows lack of non-specific staining on stromal cells in general.