Table I.
Effects of Siglec-11 expression on cytokine secretion by human ovarian stromal fibroblasts
| Cytokine | Siglec-11 (mean ± SD) | Control (mean ± SD) | Fold change | P-value |
|---|---|---|---|---|
| G-CSF | 0.01 ± 0.01 | 0.01 ± 0.01 | 0.96 | 0.96 |
| GM-CSF | 0.05 ± 0.03 | 0.05 ± 0.03 | 1.04 | 0.69 |
| GRO | 0.92 ± 0.17 | 0.95 ± 0.04 | 0.98 | 0.81 |
| GRO-α | 0.96 ± 0.19 | 0.59 ± 0.27 | 1.61 | 0.01* |
| IFN-γ | 0.03 ± 0.02 | 0.03 ± 0.02 | 0.85 | 0.69 |
| IL-10 | 0.24 ± 0.03 | 0.18 ± 0.01 | 1.32 | 0.03* |
| IL-13 | 0.02 ± 0.00 | 0.01 ± 0.01 | 1.66 | 0.41 |
| IL-15 | 0.01 ± 0.01 | 0.00 ± 0.01 | 1.29 | 0.69 |
| IL-1α | 0.07 ± 0.03 | 0.06 ± 0.02 | 1.19 | 0.60 |
| IL-2 | 0.01 ± 0.01 | 0.00 ± 0.01 | 2.61 | 0.47 |
| IL-3 | 0.03 ± 0.01 | 0.04 ± 0.01 | 0.75 | 0.17 |
| IL-5 | 0.00 ± 0.01 | 0.02 ± 0.01 | 0.04 | 0.05 |
| IL-6 | 1.03 ± 0.03 | 0.87 ± 0.13 | 1.18 | 0.08 |
| IL-7 | 0.12 ± 0.08 | 0.07 ± 0.07 | 1.68 | 0.01* |
| IL-8 | 0.86 ± 0.14 | 0.99 ± 0.07 | 0.87 | 0.27 |
| MCP-1 | 0.85 ± 0.08 | 0.88 ± 0.15 | 0.96 | 0.75 |
| MCP-2 | −0.02 ± 0.08 | 0.00 ± 0.01 | −4.89 | 0.65 |
| MCP-3 | 0.02 ± 0.03 | 0.00 ± 0.01 | 9.47 | 0.10 |
| MIG | 0.05 ± 0.03 | 0.03 ± 0.03 | 1.61 | 0.21 |
| RANTES | 0.31 ± 0.05 | 0.31 ± 0.03 | 0.99 | 0.77 |
| TGF-β1 | 0.04 ± 0.02 | 0.01 ± 0.01 | 3.71 | 0.01* |
| TNF-α | 0.16 ± 0.04 | 0.11 ± 0.04 | 1.44 | 0.02* |
| TNF-β | 0.04 ± 0.02 | 0.02 ± 0.01 | 1.71 | 0.12 |
G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; IFN, interferon; MCP, monocyte chemoattractant protein; MIG, monokine Induced by Interferon-gamma; RANTES, regulated upon activation, normal T cell expressed and secreted.
Human ovarian stromal fibroblast primary cell line HFN0402 was stably transfected in two independent experiments with Siglec-11-pcDNA3.1 or with vector only. In each case, selection was applied with G418 for 3 weeks. Collected supernatant from each transfected cell pool was assayed for various cytokine secretions in duplicate using Raybio human cytokine antibody array I (Raybiotech). Signal value for each cytokine was normalized by dividing with the average value of positive controls on each block. The mean and the standard deviation are derived from the reads (one read is the average of the duplicate) of two independent assays. Two-tailed P-values are estimated from a t-test.
*P < 0.05.