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. 1989 Feb;57(2):656–659. doi: 10.1128/iai.57.2.656-659.1989

Structural analysis of lipopolysaccharides from Eikenella corrodens by use of murine monoclonal antibodies.

T Kato 1, I Takazoe 1, K Okuda 1
PMCID: PMC313151  PMID: 2643582

Abstract

The structure of lipopolysaccharides (LPSs) of Eikenella corrodens was analyzed with prepared murine monoclonal antibodies. A common core epitope was found in three of seven LPS preparations from E. corrodens strains and Fusobacterium nucleatum ATCC 25586. Four E. corrodens LPSs were found to possess an O-side-chain epitope which cross-reacted with LPSs from Fusobacterium necrophorum ATCC 25286 and Capnocytophaga ochracea M-12. Lipid A of E. corrodens LPS shared an epitope common among LPSs from various gram-negative rods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apicella M. A., Gagliardi N. C. Antigenic heterogeneity of the non-serogroup antigen structure of Neisseria gonorrhoeae lipopolysaccharides. Infect Immun. 1979 Dec;26(3):870–874. doi: 10.1128/iai.26.3.870-874.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Apicella M. A. Serogrouping of Neisseria gonorrhoeae: identification of four immunologically distinct acidic polysaccharides. J Infect Dis. 1976 Oct;134(4):377–383. doi: 10.1093/infdis/134.4.377. [DOI] [PubMed] [Google Scholar]
  3. Behling U. H., Pham P. H., Nowotny A. Biological activity of the slime and endotoxin of the periodontopathic organism Eikenella corrodens. Infect Immun. 1979 Nov;26(2):580–584. doi: 10.1128/iai.26.2.580-584.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dahlén G., Mattsby-Baltzer I. Lipid A in anaerobic bacteria. Infect Immun. 1983 Jan;39(1):466–468. doi: 10.1128/iai.39.1.466-468.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hofstad T. Chemical characteristics of Bacteroides melaninogenicus endotoxin. Arch Oral Biol. 1968 Sep;13(9):1149–1155. doi: 10.1016/0003-9969(68)90067-8. [DOI] [PubMed] [Google Scholar]
  7. Johnson D. A., Behling U. H., Listgarten M., Nowotny A. Role of bacterial products in periodontitis: humoral immune response to Eikenella corrodens. Infect Immun. 1978 Nov;22(2):382–386. doi: 10.1128/iai.22.2.382-386.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kirkland T. N., Colwell D. E., Michalek S. M., McGhee J. R., Ziegler E. J. Analysis of the fine specificity and cross-reactivity of monoclonal anti-lipid A antibodies. J Immunol. 1986 Dec 1;137(11):3614–3619. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Listgarten M. A., Johnson D., Nowotny A., Tanner A. C., Socransky S. S. Histopathology of periodontal disease in gnotobiotic rats monoinfected with Eikenella corrodens. J Periodontal Res. 1978 Mar;13(2):134–148. doi: 10.1111/j.1600-0765.1978.tb00162.x. [DOI] [PubMed] [Google Scholar]
  11. Mashimo J., Yoshida M., Ikeuchi K., Hata S., Arata S., Kasai N., Okuda K., Takazoe I. Fatty acid composition and Shwartzman activity of lipopolysaccharides from oral bacteria. Microbiol Immunol. 1985;29(5):395–403. doi: 10.1111/j.1348-0421.1985.tb00840.x. [DOI] [PubMed] [Google Scholar]
  12. Morrison D. C., Jacobs D. M. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry. 1976 Oct;13(10):813–818. doi: 10.1016/0019-2791(76)90181-6. [DOI] [PubMed] [Google Scholar]
  13. Naito Y., Okuda K., Kato T., Takazoe I. Monoclonal antibodies against surface antigens of Bacteroides gingivalis. Infect Immun. 1985 Oct;50(1):231–235. doi: 10.1128/iai.50.1.231-235.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Newman M. G., Socransky S. S., Savitt E. D., Propas D. A., Crawford A. Studies of the microbiology of periodontosis. J Periodontol. 1976 Jul;47(7):373–379. doi: 10.1902/jop.1976.47.7.373. [DOI] [PubMed] [Google Scholar]
  15. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  16. Okuda K., Kato T. Hemagglutinating activity of lipopolysaccharides from subgingival plaque bacteria. Infect Immun. 1987 Dec;55(12):3192–3196. doi: 10.1128/iai.55.12.3192-3196.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Okuda K., Naito Y., Ohta K., Fukumoto Y., Kimura Y., Ishikawa I., Kinoshita S., Takazoe I. Bacteriological study of periodontal lesions in two sisters with juvenile periodontitis and their mother. Infect Immun. 1984 Jul;45(1):118–121. doi: 10.1128/iai.45.1.118-121.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Perez-Perez G. I., Hopkins J. A., Blaser M. J. Lipopolysaccharide structures in Enterobacteriaceae, Pseudomonas aeruginosa, and Vibrio cholerae are immunologically related to Campylobacter spp. Infect Immun. 1986 Jan;51(1):204–208. doi: 10.1128/iai.51.1.204-208.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Progulske A., Holt S. C. Isolation and characterization of the outer membrane and lipopolysaccharide from Eikenella corrodens. Infect Immun. 1984 Jan;43(1):166–177. doi: 10.1128/iai.43.1.166-177.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Progulske A., Mishell R., Trummel C., Holt S. C. Biological activities of Eikenella corrodens outer membrane and lipopolysaccharide. Infect Immun. 1984 Jan;43(1):178–182. doi: 10.1128/iai.43.1.178-182.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sasaki S. Biological activity of lipopolysaccharides isolated from bacteria in human periodontal lesions. Bull Tokyo Dent Coll. 1979 Nov;20(4):159–174. [PubMed] [Google Scholar]
  22. Shiozu J. [Biological, genetic and antigenic characteristics of Eikenella corrodens]. Shikwa Gakuho. 1985 Feb;85(2):121–134. [PubMed] [Google Scholar]
  23. Socransky S. S. Microbiology of periodontal disease -- present status and future considerations. J Periodontol. 1977 Sep;48(9):497–504. doi: 10.1902/jop.1977.48.9.497. [DOI] [PubMed] [Google Scholar]
  24. Tanner A. C., Haffer C., Bratthall G. T., Visconti R. A., Socransky S. S. A study of the bacteria associated with advancing periodontitis in man. J Clin Periodontol. 1979 Oct;6(5):278–307. doi: 10.1111/j.1600-051x.1979.tb01931.x. [DOI] [PubMed] [Google Scholar]
  25. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]

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