Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1989 Mar;57(3):739–744. doi: 10.1128/iai.57.3.739-744.1989

Immunoprophylaxis of Chlamydia trachomatis lymphogranuloma venereum pneumonitis in mice by oral immunization.

Z D Cui 1, L J LaScolea Jr 1, J Fisher 1, P L Ogra 1
PMCID: PMC313171  PMID: 2917786

Abstract

Groups of BALB/c mice were orally immunized with chlamydiae and challenged intranasally to determine whether oral immunization offers protection against pulmonary disease and to characterize the nature and kinetics of the chlamydial antibody response in the lung and other mucosal sites. Protection by oral immunization from chlamydial lung disease was demonstrated by lack of replication of the organism and the lack of chlamydial antigen in lung tissue. The chlamydial immunoglobulin A (IgA) antibody response was present at all body sites, reaching peak levels in the lung as well as in the serum. Classical IgA booster effect kinetics was observed after intranasal challenge, especially in the lung. Specific IgG antibody was detected at all body sites but at lower levels. Furthermore, animals immunized orally had no pneumonic process, as determined by histopathology. These studies also suggest that passively acquired specific serum IgG antibody may not significantly influence the course of mucosal replication of the organism. These observations indicate that oral immunization activating the gut-associated lymphoid tissue system gave total protection against chlamydial lung disease, suggesting migration of immunologically competent cells from the intestine to the lung.

Full text

PDF
739

Images in this article

741Image
on p.741

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alani M. D., Darougar S., Burns D. C., Thin R. N., Dunn H. Isolation of Chlamydia trachomatis from the male urethra. Br J Vener Dis. 1977 Apr;53(2):88–92. doi: 10.1136/sti.53.2.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barenfanger J., MacDonald A. B. The role of immunoglobulin in the neutralization of trachoma infectivity. J Immunol. 1974 Nov;113(5):1607–1617. [PubMed] [Google Scholar]
  3. Brunham R. C., Kuo C. C., Cles L., Holmes K. K. Correlation of host immune response with quantitative recovery of Chlamydia trachomatis from the human endocervix. Infect Immun. 1983 Mar;39(3):1491–1494. doi: 10.1128/iai.39.3.1491-1494.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caldwell H. D., Kromhout J., Schachter J. Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis. Infect Immun. 1981 Mar;31(3):1161–1176. doi: 10.1128/iai.31.3.1161-1176.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Frommell G. T., Rothenberg R., Wang S., McIntosh K. Chlamydial infection of mothers and their infants. J Pediatr. 1979 Jul;95(1):28–32. doi: 10.1016/s0022-3476(79)80077-3. [DOI] [PubMed] [Google Scholar]
  6. Hammerschlag M. R., Anderka M., Semine D. Z., McComb D., McCormack W. M. Prospective study of maternal and infantile infection with Chlamydia trachomatis. Pediatrics. 1979 Aug;64(2):142–148. [PubMed] [Google Scholar]
  7. Hammerschlag M. R. Chlamydial pneumonia in infants. N Engl J Med. 1978 May 11;298(19):1083–1084. doi: 10.1056/NEJM197805112981912. [DOI] [PubMed] [Google Scholar]
  8. Howard L. V., O'Leary M. P., Nichols R. L. Animal model studies of genital chlamydial infections. Immunity to re-infection with guinea-pig inclusion conjunctivitis agent in the urethra and eye of male guinea-pigs. Br J Vener Dis. 1976 Aug;52(4):261–265. doi: 10.1136/sti.52.4.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson A. P. Pathogenesis and immunology of chlamydial infections of the genital tract. Rev Infect Dis. 1985 Nov-Dec;7(6):741–745. doi: 10.1093/clinids/7.6.741. [DOI] [PubMed] [Google Scholar]
  10. La Scolea L. J., Jr, Keddell J. E. Efficacy of various cell culture procedures for detection of Chlamydia trachomatis and applicability to diagnosis of pediatric infections. J Clin Microbiol. 1981 Apr;13(4):705–708. doi: 10.1128/jcm.13.4.705-708.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lamont H. C., Semine D. Z., Leveille C., Nichols R. L. Immunity to vaginal reinfection in female guinea pigs infected sexually with Chlamydia of guinea pig inclusion conjunctivitis. Infect Immun. 1978 Mar;19(3):807–813. doi: 10.1128/iai.19.3.807-813.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McComb D. E., Nichols R. L., Semine D. Z., Evrard J. R., Alpert S., Crockett V. A., Rosner B., Zinner S. H., McCormack W. M. Chlamydia trachomatis in women: antibody in cervical secretions as a possible indicator of genital infection. J Infect Dis. 1979 Jun;139(6):628–633. doi: 10.1093/infdis/139.6.628. [DOI] [PubMed] [Google Scholar]
  13. McCormack W. M., Alpert S., McComb D. E., Nichols R. L., Semine D. Z., Zinner S. H. Fifteen-month follow-up study of women infected with Chlamydia trachomatis. N Engl J Med. 1979 Jan 18;300(3):123–125. doi: 10.1056/NEJM197901183000305. [DOI] [PubMed] [Google Scholar]
  14. Meyer K. F. The host spectrum of psittacosis-lymphogranuloma venereum (PL) agents. Am J Ophthalmol. 1967 May;63(5 Suppl):1225–1246. doi: 10.1016/0002-9394(67)94105-0. [DOI] [PubMed] [Google Scholar]
  15. Murray E. S., Charbonnet L. T., MacDonald A. B. Immunity to chlamydial infections of the eye. I. The role of circulatory and secretory antibodies in resistance to reinfection with guinea pig inclusion conjunctivitis. J Immunol. 1973 Jun;110(6):1518–1525. [PubMed] [Google Scholar]
  16. Nichols R. L., Murray E. S., Nisson P. E. Use of enteric vaccines in protection against chlamydial infections of the genital tract and the eye of guinea pigs. J Infect Dis. 1978 Dec;138(6):742–746. doi: 10.1093/infdis/138.6.742. [DOI] [PubMed] [Google Scholar]
  17. Rosales S. V., Lascolea L. J., Jr, Ogra P. L. Development of respiratory mucosal tolerance during Haemophilus influenzae type B infection in infancy. J Immunol. 1984 Mar;132(3):1517–1521. [PubMed] [Google Scholar]
  18. Schachter J. Chlamydial infections (third of three parts). N Engl J Med. 1978 Mar 9;298(10):540–549. doi: 10.1056/NEJM197803092981005. [DOI] [PubMed] [Google Scholar]
  19. Schachter J., Grossman M., Holt J., Sweet R., Goodner E., Mills J. Prospective study of chlamydial infection in neonates. Lancet. 1979 Aug 25;2(8139):377–380. doi: 10.1016/s0140-6736(79)90400-8. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES