Abstract
Campylobacter pylori was identified with immunoperoxidase staining and a mouse monoclonal antibody directed against C. pylori in gastric biopsy specimens from 24 patients with gastritis. C. pylori was not found in gastric biopsy specimens from six subjects with histologically normal mucosa. The monoclonal antibody, which was reactive with a surface protein of approximately 20 kilodaltons, was found to be specific for C. pylori, and the immunoperoxidase staining proved to be more sensitive and rapid than culture in detecting the organism. In the tissue specimens where C. pylori was detected with the monoclonal antibody, there was a strong expression of class II transplantation antigens on the epithelial cells and an increased number of T lymphocytes. These findings indicate that C. pylori may initiate local immune responses.
Full text
PDF





Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barclay A. N., Mason D. W. Induction of Ia antigen in rat epidermal cells and gut epithelium by immunological stimuli. J Exp Med. 1982 Dec 1;156(6):1665–1676. doi: 10.1084/jem.156.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Campylobacter-like organisms in the stomach of patients and healthy individuals. Lancet. 1984 Jun 16;1(8390):1348–1349. [PubMed] [Google Scholar]
- Coghlan J. G., Gilligan D., Humphries H., McKenna D., Dooley C., Sweeney E., Keane C., O'Morain C. Campylobacter pylori and recurrence of duodenal ulcers--a 12-month follow-up study. Lancet. 1987 Nov 14;2(8568):1109–1111. doi: 10.1016/s0140-6736(87)91545-5. [DOI] [PubMed] [Google Scholar]
- Curry A., Jones D. M., Eldridge J. Spiral organisms in the baboon stomach. Lancet. 1987 Sep 12;2(8559):634–635. doi: 10.1016/s0140-6736(87)93032-7. [DOI] [PubMed] [Google Scholar]
- Engstrand L., Pählson C., Gustavsson S., Schwan A. Monoclonal antibodies for rapid identification of Campylobacter pyloridis. Lancet. 1986 Dec 13;2(8520):1402–1403. doi: 10.1016/s0140-6736(86)92049-0. [DOI] [PubMed] [Google Scholar]
- Forsum U., Claesson K., Hjelm E., Karlsson-Parra A., Klareskog L., Scheynius A., Tjernlund U. Class II transplantation antigens: distribution in tissues and involvement in disease. Scand J Immunol. 1985 May;21(5):389–396. doi: 10.1111/j.1365-3083.1985.tb01823.x. [DOI] [PubMed] [Google Scholar]
- Forsum U., Claesson K., Jonsson R., Karlsson-Parra A., Klareskog L., Scheynius A., Tjernlund U. Differential tissue distribution of HLA-DR, -DP and -DQ antigens. Adv Exp Med Biol. 1987;216A:233–239. doi: 10.1007/978-1-4684-5344-7_27. [DOI] [PubMed] [Google Scholar]
- Gustavsson S., Phillips S. F., Malagelada J. R., Rosenblatt J. E. Assessment of Campylobacter-like organisms in the postoperative stomach, iatrogenic gastritis, and chronic gastroduodenal diseases: preliminary observations. Mayo Clin Proc. 1987 Apr;62(4):265–268. doi: 10.1016/s0025-6196(12)61902-8. [DOI] [PubMed] [Google Scholar]
- Hamilton I., O'Connor H. J., Wood N. C., Bradbury I., Axon A. T. Healing and recurrence of duodenal ulcer after treatment with tripotassium dicitrato bismuthate (TDB) tablets or cimetidine. Gut. 1986 Jan;27(1):106–110. doi: 10.1136/gut.27.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hjelm E. M. Local cellular immune response in ascending urinary tract infection: occurrence of T-cells, immunoglobulin-producing cells, and Ia-expressing cells in rat urinary tract tissue. Infect Immun. 1984 Jun;44(3):627–632. doi: 10.1128/iai.44.3.627-632.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hornick R. B. Peptic ulcer disease: a bacterial infection? N Engl J Med. 1987 Jun 18;316(25):1598–1600. doi: 10.1056/NEJM198706183162509. [DOI] [PubMed] [Google Scholar]
- Kaplow I. S. Letter: Substitute for benzidine in myeloperoxidase stains. Am J Clin Pathol. 1975 Mar;63(3):451–451. doi: 10.1093/ajcp/63.3.451. [DOI] [PubMed] [Google Scholar]
- Klareskog L., Forsum U., Peterson P. A. Hormonal regulation of the expression of Ia antigens on mammary gland epithelium. Eur J Immunol. 1980 Dec;10(12):958–963. doi: 10.1002/eji.1830101212. [DOI] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Marshall B. J., Armstrong J. A., McGechie D. B., Glancy R. J. Attempt to fulfil Koch's postulates for pyloric Campylobacter. Med J Aust. 1985 Apr 15;142(8):436–439. doi: 10.5694/j.1326-5377.1985.tb113443.x. [DOI] [PubMed] [Google Scholar]
- Marshall B. J. Campylobacter pyloridis and gastritis. J Infect Dis. 1986 Apr;153(4):650–657. doi: 10.1093/infdis/153.4.650. [DOI] [PubMed] [Google Scholar]
- Marshall B. J., McGechie D. B., Rogers P. A., Glancy R. J. Pyloric Campylobacter infection and gastroduodenal disease. Med J Aust. 1985 Apr 15;142(8):439–444. doi: 10.5694/j.1326-5377.1985.tb113444.x. [DOI] [PubMed] [Google Scholar]
- McNulty C. A., Wise R. Rapid diagnosis of Campylobacter-associated gastritis. Lancet. 1985 Jun 22;1(8443):1443–1444. doi: 10.1016/s0140-6736(85)91865-3. [DOI] [PubMed] [Google Scholar]
- Pober J. S., Gimbrone M. A., Jr, Cotran R. S., Reiss C. S., Burakoff S. J., Fiers W., Ault K. A. Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon. J Exp Med. 1983 Apr 1;157(4):1339–1353. doi: 10.1084/jem.157.4.1339. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Price A. B., Levi J., Dolby J. M., Dunscombe P. L., Smith A., Clark J., Stephenson M. L. Campylobacter pyloridis in peptic ulcer disease: microbiology, pathology, and scanning electron microscopy. Gut. 1985 Nov;26(11):1183–1188. doi: 10.1136/gut.26.11.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Påhlson C., Hallén A., Forsum U. Curved rods related to Mobiluncus--phenotypes as defined by monoclonal antibodies. Acta Pathol Microbiol Immunol Scand B. 1986 Jun;94(3):117–125. doi: 10.1111/j.1699-0463.1986.tb03030.x. [DOI] [PubMed] [Google Scholar]
- Rathbone B. J., Wyatt J. I., Worsley B. W., Shires S. E., Trejdosiewicz L. K., Heatley R. V., Losowsky M. S. Systemic and local antibody responses to gastric Campylobacter pyloridis in non-ulcer dyspepsia. Gut. 1986 Jun;27(6):642–647. doi: 10.1136/gut.27.6.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scheynius A., Johansson C., van der Meide P. H. In vivo induction of Ia antigens on rat keratinocytes by gamma-interferon. Br J Dermatol. 1986 Nov;115(5):543–549. doi: 10.1111/j.1365-2133.1986.tb05763.x. [DOI] [PubMed] [Google Scholar]
- Selby W. S., Janossy G., Bofill M., Jewell D. P. Lymphocyte subpopulations in the human small intestine. The findings in normal mucosa and in the mucosa of patients with adult coeliac disease. Clin Exp Immunol. 1983 Apr;52(1):219–228. [PMC free article] [PubMed] [Google Scholar]
- Steeg P. S., Moore R. N., Johnson H. M., Oppenheim J. J. Regulation of murine macrophage Ia antigen expression by a lymphokine with immune interferon activity. J Exp Med. 1982 Dec 1;156(6):1780–1793. doi: 10.1084/jem.156.6.1780. [DOI] [PMC free article] [PubMed] [Google Scholar]