Abstract
Addition of murine recombinant gamma interferon (IFN-gamma) to mouse fibroblast cultures infected with Chlamydia psittaci was found to induce a cytotoxic response that was dependent on the concentration of IFN-gamma added and the multiplicity of infection given. No cytotoxicity was observed for uninfected cells treated with IFN-gamma, nor did infection alone elicit cytotoxicity. Cytotoxicity was detected only if IFN-gamma was present for at least the first 18 h of a 30-h incubation period. Cytotoxic activity was not observed when infected cells were treated with 50 micrograms of chloramphenicol per ml, a drug which inhibits differentiation of infectious elementary bodies to noninfectious reticulate bodies. Cytotoxic activity was restored if addition of chloramphenicol was delayed until 18 h postinfection. Addition of 100 U of penicillin per ml to infected host cells reduced but did not abolish cytotoxic activity. Treatment of host cells with as little as 0.2 microgram of cycloheximide per ml inhibited cytotoxicity without interfering with chlamydial growth. When addition of cycloheximide was delayed until 12 h after infection and IFN-gamma treatment, cytotoxicity was restored. These data indicate that IFN-gamma functions as a cytotoxic cytokine against chlamydia-infected fibroblasts. Cytotoxicity was found to be dependent on chlamydial multiplicity of infection, differentiation of chlamydiae to the metabolically active form, and host cell protein synthesis.
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- Byrne G. I., Grubbs B., Dickey T. J., Schachter J., Williams D. M. Interferon in recovery from pneumonia due to Chlamydia trachomatis in the mouse. J Infect Dis. 1987 Dec;156(6):993–996. doi: 10.1093/infdis/156.6.993. [DOI] [PubMed] [Google Scholar]
- Byrne G. I., Grubbs B., Marshall T. J., Schachter J., Williams D. M. Gamma interferon-mediated cytotoxicity related to murine Chlamydia trachomatis infection. Infect Immun. 1988 Aug;56(8):2023–2027. doi: 10.1128/iai.56.8.2023-2027.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Byrne G. I., Krueger D. A. In vitro expression of factor-mediated cytotoxic activity generated during the immune response to Chlamydia in the mouse. J Immunol. 1985 Jun;134(6):4189–4193. [PubMed] [Google Scholar]
- Byrne G. I., Krueger D. A. Lymphokine-mediated inhibition of Chlamydia replication in mouse fibroblasts is neutralized by anti-gamma interferon immunoglobulin. Infect Immun. 1983 Dec;42(3):1152–1158. doi: 10.1128/iai.42.3.1152-1158.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hatch T. P. Competition between Chlamydia psittaci and L cells for host isoleucine pools: a limiting factor in chlamydial multiplication. Infect Immun. 1975 Jul;12(1):211–220. doi: 10.1128/iai.12.1.211-220.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsumoto A., Manire G. P. Electron microscopic observations on the effects of penicillin on the morphology of Chlamydia psittaci. J Bacteriol. 1970 Jan;101(1):278–285. doi: 10.1128/jb.101.1.278-285.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murray H. W. Interferon-gamma, the activated macrophage, and host defense against microbial challenge. Ann Intern Med. 1988 Apr;108(4):595–608. doi: 10.7326/0003-4819-108-4-595. [DOI] [PubMed] [Google Scholar]
- Rothermel C. D., Rubin B. Y., Murray H. W. Gamma-interferon is the factor in lymphokine that activates human macrophages to inhibit intracellular Chlamydia psittaci replication. J Immunol. 1983 Nov;131(5):2542–2544. [PubMed] [Google Scholar]
- Shemer Y., Sarov I. Inhibition of growth of Chlamydia trachomatis by human gamma interferon. Infect Immun. 1985 May;48(2):592–596. doi: 10.1128/iai.48.2.592-596.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zhong G. M., Peterson E. M., Czarniecki C. W., de la Maza L. M. Recombinant murine gamma interferon inhibits Chlamydia trachomatis serovar L1 in vivo. Infect Immun. 1988 Jan;56(1):283–286. doi: 10.1128/iai.56.1.283-286.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de la Maza L. M., Peterson E. M., Fennie C. W., Czarniecki C. W. The anti-chlamydial and anti-proliferative activities of recombinant murine interferon-gamma are not dependent on tryptophan concentrations. J Immunol. 1985 Dec;135(6):4198–4200. [PubMed] [Google Scholar]
- de la Maza L. M., Peterson E. M., Goebel J. M., Fennie C. W., Czarniecki C. W. Interferon-induced inhibition of Chlamydia trachomatis: dissociation from antiviral and antiproliferative effects. Infect Immun. 1985 Mar;47(3):719–722. doi: 10.1128/iai.47.3.719-722.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]