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. 1989 Apr;57(4):1235–1239. doi: 10.1128/iai.57.4.1235-1239.1989

Bacteriologic and serologic findings in experimental pyelonephritis caused by Ureaplasma urealyticum.

W J Pickering 1, D F Birch 1
PMCID: PMC313255  PMID: 2925249

Abstract

A challenge procedure based on reflux of Ureaplasma urealyticum to the rat kidney was used to examine the disease-causing potential of this species for the upper urinary tract. Renal infection was detected 8 days after challenge in 90 to 100% of animals exposed to 10(7) ureaplasmas but was not detected in animals exposed to fewer than 10(6) microorganisms. Viable microorganisms were recoverable from more than 60% of infected animals throughout a 24-week follow-up period of study. Animals with persistent infection exhibited a humoral response involving immunoglobulin M and immunoglobulin G antibody. These results show that U. urealyticum is capable of giving rise to chronic infection in the kidneys of rats following challenge via the ascending route.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birch D. F., Fairley K. F., Pavillard R. E. Unconventional bacteria in urinary tract disease: Ureaplasma urealyticum. Kidney Int. 1981 Jan;19(1):58–64. doi: 10.1038/ki.1981.7. [DOI] [PubMed] [Google Scholar]
  2. Ford D. K., MacDonald J. Influence of urea on the growth of T-strain mycoplasmas. J Bacteriol. 1967 May;93(5):1509–1512. doi: 10.1128/jb.93.5.1509-1512.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hewish M. J., Birch D. F., Fairley K. F. Ureaplasma urealyticum serotypes in urinary tract disease. J Clin Microbiol. 1986 Jan;23(1):149–154. doi: 10.1128/jcm.23.1.149-154.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kenny G. E., Cartwright F. D. Effect of urea concentration on growth of Ureaplasma urealyticum (T-strain mycoplasma). J Bacteriol. 1977 Oct;132(1):144–150. doi: 10.1128/jb.132.1.144-150.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Mårdh P. A., Taylor-Robinson D. The differential effect of lysolecithin on mycoplasmas and acholeplasmas. Med Microbiol Immunol. 1973 Mar 8;158(3):219–226. doi: 10.1007/BF02120557. [DOI] [PubMed] [Google Scholar]
  7. Robertson J. A., Stemke G. W. Expanded serotyping scheme for Ureaplasma urealyticum strains isolated from humans. J Clin Microbiol. 1982 May;15(5):873–878. doi: 10.1128/jcm.15.5.873-878.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SHEPARD M. C., LUNCEFORD C. D. EFFECT OF PH ON HUMAN MYCOPLASMA STRAINS. J Bacteriol. 1965 Feb;89:265–270. doi: 10.1128/jb.89.2.265-270.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sayed I. A., Kenny G. E. Effect of ammonium ion concentration and osmotic pressure on growth of Ureaplasma urealyticum (T-strain mycoplasma). J Bacteriol. 1978 Jun;134(3):967–972. doi: 10.1128/jb.134.3.967-972.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shepard M. C., Lunceford C. D. Serological typing of Ureaplasma urealyticum isolates from urethritis patients by an agar growth inhibition method. J Clin Microbiol. 1978 Nov;8(5):566–574. doi: 10.1128/jcm.8.5.566-574.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Thomsen A. C. Occurrence of mycoplasmas in urinary tracts of patients with acute pyelonephritis. J Clin Microbiol. 1978 Jul;8(1):84–88. doi: 10.1128/jcm.8.1.84-88.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thomsen A. C. The isolation of Mycoplasma primatum during an autopsy study of the mycoplasma flora of the human urinary tract. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Oct;82B(5):653–656. doi: 10.1111/j.1699-0463.1974.tb00232.x. [DOI] [PubMed] [Google Scholar]
  13. Turunen H., Leinikki P., Jansson E. Serological characterisation of Ureaplasma urealyticum strains by enzyme-linked immunosorbent assay (ELISA). J Clin Pathol. 1982 Apr;35(4):439–443. doi: 10.1136/jcp.35.4.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wiley C. A., Quinn P. A. Enzyme-linked immunosorbent assay for detection of specific antibodies to Ureaplasma urealyticum serotypes. J Clin Microbiol. 1984 Mar;19(3):421–426. doi: 10.1128/jcm.19.3.421-426.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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