Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1989 Apr;57(4):1327–1331. doi: 10.1128/iai.57.4.1327-1331.1989

Actinomyces viscosus fibril antigens detected by immunogold electron microscopy.

R P Ellen 1, I A Buivids 1, J R Simardone 1
PMCID: PMC313271  PMID: 2564376

Abstract

Strains representing taxonomic clusters of Actinomyces viscosus and Actinomyces naeslundii were studied by indirect immunogold electron microscopy with either monospecific anti-type 1 and anti-type 2 rabbit antibodies or species-specific monoclonal antibodies. The monoclonal and anti-type 2 antibodies localized on long fibrils, whereas the anti-type 1 antibodies mostly localized close to the cell body or on shorter appendages.

Full text

PDF
1327

Images in this article

1328Image
on p.1328
1329Image
on p.1329
1329Image
on p.1329
1330Image
on p.1330

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brennan M. J., Joralmon R. A., Cisar J. O., Sandberg A. L. Binding of Actinomyces naeslundii to glycosphingolipids. Infect Immun. 1987 Feb;55(2):487–489. doi: 10.1128/iai.55.2.487-489.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cisar J. O., David V. A., Curl S. H., Vatter A. E. Exclusive presence of lactose-sensitive fimbriae on a typical strain (WVU45) of Actinomyces naeslundii. Infect Immun. 1984 Nov;46(2):453–458. doi: 10.1128/iai.46.2.453-458.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cisar J. O., Sandberg A. L., Mergenhagen S. E. The function and distribution of different fimbriae on strains of Actinomyces viscosus and Actinomyces naeslundii. J Dent Res. 1984 Mar;63(3):393–396. doi: 10.1177/00220345840630030701. [DOI] [PubMed] [Google Scholar]
  4. Cisar J. O., Vatter A. E., Clark W. B., Curl S. H., Hurst-Calderone S., Sandberg A. L. Mutants of Actinomyces viscosus T14V lacking type 1, type 2, or both types of fimbriae. Infect Immun. 1988 Nov;56(11):2984–2989. doi: 10.1128/iai.56.11.2984-2989.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clark W. B., Lane M. D., Beem J. E., Bragg S. L., Wheeler T. T. Relative hydrophobicities of Actinomyces viscosus and Actinomyces naeslundii strains and their adsorption to saliva-treated hydroxyapatite. Infect Immun. 1985 Mar;47(3):730–736. doi: 10.1128/iai.47.3.730-736.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark W. B., Wheeler T. T., Lane M. D., Cisar J. O. Actinomyces adsorption mediated by type-1 fimbriae. J Dent Res. 1986 Sep;65(9):1166–1168. doi: 10.1177/00220345860650091001. [DOI] [PubMed] [Google Scholar]
  7. Costello A. H., Cisar J. O., Kolenbrander P. E., Gabriel O. Neuraminidase-dependent hamagglutination of human erythrocytes by human strains of Actinomyces viscosus and Actinomyces naeslundii. Infect Immun. 1979 Nov;26(2):563–572. doi: 10.1128/iai.26.2.563-572.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ellen R. P., Fillery E. D., Chan K. H., Grove D. A. Sialidase-enhanced lectin-like mechanism for Actinomyces viscosus and Actinomyces naeslundii hemagglutination. Infect Immun. 1980 Feb;27(2):335–343. doi: 10.1128/iai.27.2.335-343.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ellen R. P., Fillery E. D., Seckington K. Infrequent polysaccharide cohesion among Actinomyces viscosus and Actinomyces naeslundii fresh isolates of human origin. J Periodontal Res. 1983 Jan;18(1):50–55. doi: 10.1111/j.1600-0765.1983.tb00334.x. [DOI] [PubMed] [Google Scholar]
  10. Ellen R. P., Grove D. A. Assignment of Actinomyces viscosus and Actinomyces naeslundii strains to numerical taxonomy clusters by immunofluorescence based on antifibril antisera. J Clin Microbiol. 1985 May;21(5):850–853. doi: 10.1128/jcm.21.5.850-853.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ellen R. P., Sivendra R. In vitro attachment, salivary agglutination, and surface fibril density of fresh Actinomyces isolates from two distinct oral surfaces. J Dent Res. 1985 May;64(5):799–803. doi: 10.1177/00220345850640050301. [DOI] [PubMed] [Google Scholar]
  12. Fillery E. D., Bowden G. H., Hardie J. M. A comparison of strains of bacteria designated Actinomyces viscosus and Actinomyces naeslundii. Caries Res. 1978;12(6):299–312. doi: 10.1159/000260349. [DOI] [PubMed] [Google Scholar]
  13. Firtel M., Fillery E. D. Distribution of antigenic determinants between Actinomyces viscosus and Actinomyces naeslundii. J Dent Res. 1988 Jan;67(1):15–20. doi: 10.1177/00220345880670010101. [DOI] [PubMed] [Google Scholar]
  14. Kolenbrander P. E., Williams B. L. Lactose-reversible coaggregation between oral actinomycetes and Streptococcus sanguis. Infect Immun. 1981 Jul;33(1):95–102. doi: 10.1128/iai.33.1.95-102.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Masuda N., Ellen R. P., Fillery E. D., Grove D. A. Chemical and immunological comparison of surface fibrils of strains representing six taxonomic groups of Actinomyces viscosus and Actinomyces naeslundii. Infect Immun. 1983 Mar;39(3):1325–1333. doi: 10.1128/iai.39.3.1325-1333.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McIntire F. C., Crosby L. K., Vatter A. E. Inhibitors of coaggregation between Actinomyces viscosus T14V and Streptococcus sanguis 34: beta-galactosides, related sugars, and anionic amphipathic compounds. Infect Immun. 1982 Apr;36(1):371–378. doi: 10.1128/iai.36.1.371-378.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mergenhagen S. E., Sandberg A. L., Chassy B. M., Brennan M. J., Yeung M. K., Donkersloot J. A., Cisar J. O. Molecular basis of bacterial adhesion in the oral cavity. Rev Infect Dis. 1987 Sep-Oct;9 (Suppl 5):S467–S474. doi: 10.1093/clinids/9.supplement_5.s467. [DOI] [PubMed] [Google Scholar]
  18. Revis G. J., Vatter A. E., Crowle A. J., Cisar J. O. Antibodies against the Ag2 fimbriae of Actinomyces viscosus T14V inhibit lactose-sensitive bacterial adherence. Infect Immun. 1982 Jun;36(3):1217–1222. doi: 10.1128/iai.36.3.1217-1222.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sandberg A. L., Mudrick L. L., Cisar J. O., Brennan M. J., Mergenhagen S. E., Vatter A. E. Type 2 fimbrial lectin-mediated phagocytosis of oral Actinomyces spp. by polymorphonuclear leukocytes. Infect Immun. 1986 Nov;54(2):472–476. doi: 10.1128/iai.54.2.472-476.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Socransky S. S., Dzink J. L., Smith C. M. Chemically defined medium for oral microorganisms. J Clin Microbiol. 1985 Aug;22(2):303–305. doi: 10.1128/jcm.22.2.303-305.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES