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. 1989 May;57(5):1432–1437. doi: 10.1128/iai.57.5.1432-1437.1989

Population genetics of human and animal enteric Campylobacter strains.

M Aeschbacher 1, J C Piffaretti 1
PMCID: PMC313295  PMID: 2707852

Abstract

A total of 125 strains of Campylobacter jejuni and Campylobacter coli from human and animal hosts were analyzed by multilocus enzyme electrophoresis. Ten genetic loci were characterized by the electrophoretic mobilities of their products, and all were found to be polymorphic. The nine loci considered for genetic analysis had an average allele number of 6.9 and a mean genetic diversity of 0.634. The strains were grouped into 64 electrophoretic types (ETs) forming two principal clusters; the first cluster consisted of 50 ETs represented by 104 isolates of C.jejuni, and the second included 14 ETs represented by 21 isolates of C. coli. The genetic distance between the two species was 0.939. The data support the conclusions that (i) campylobacteriosis caused by C. jejuni and C. coli is a zoonosis, (ii) human and animal strains do not constitute subpopulations, and (iii) every animal strain may be considered a potential human pathogen. The frequency of intraspecies gene transfer seems to be moderately high whereas interspecies gene transfer is very rare at best, which suggests the existence of a biological barrier between C. jejuni and C. coli.

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Selected References

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  1. Blaser M. J., Taylor D. N., Feldman R. A. Epidemiology of Campylobacter jejuni infections. Epidemiol Rev. 1983;5:157–176. doi: 10.1093/oxfordjournals.epirev.a036256. [DOI] [PubMed] [Google Scholar]
  2. Bolton F. J., Holt A. V., Hutchinson D. N. Campylobacter biotyping scheme of epidemiological value. J Clin Pathol. 1984 Jun;37(6):677–681. doi: 10.1136/jcp.37.6.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradbury W. C., Pearson A. D., Marko M. A., Congi R. V., Penner J. L. Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis. J Clin Microbiol. 1984 Mar;19(3):342–346. doi: 10.1128/jcm.19.3.342-346.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bänffer J. R. Biotypes and serotypes of Campylobacter jejuni and Campylobacter coli strains isolated from patients, pigs and chickens in the region of Rotterdam. J Infect. 1985 May;10(3):277–281. doi: 10.1016/s0163-4453(85)92813-0. [DOI] [PubMed] [Google Scholar]
  5. Collins D. M., Ross D. E. Restriction endonuclease analysis of Campylobacter strains with particular reference to Campylobacter fetus ss. fetus. J Med Microbiol. 1984 Aug;18(1):117–124. doi: 10.1099/00222615-18-1-117. [DOI] [PubMed] [Google Scholar]
  6. Colver A. F., Pedler S. J., Hawkey P. M. Severe campylobacter infection in children. J Infect. 1985 Nov;11(3):217–220. doi: 10.1016/s0163-4453(85)93096-8. [DOI] [PubMed] [Google Scholar]
  7. Finch M. J., Riley L. W. Campylobacter infections in the United States. Results of an 11-state surveillance. Arch Intern Med. 1984 Aug;144(8):1610–1612. [PubMed] [Google Scholar]
  8. Grajewski B. A., Kusek J. W., Gelfand H. M. Development of a bacteriophage typing system for Campylobacter jejuni and Campylobacter coli. J Clin Microbiol. 1985 Jul;22(1):13–18. doi: 10.1128/jcm.22.1.13-18.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hutchinson D. N., Bolton F. J., Jones D. M., Sutcliffe E. M., Abbott J. D. Application of three typing schemes (Penner, Lior, Preston) to strains of Campylobacter spp. isolated from three outbreaks. Epidemiol Infect. 1987 Apr;98(2):139–144. doi: 10.1017/s0950268800061847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hébert G. A., Hollis D. G., Weaver R. E., Lambert M. A., Blaser M. J., Moss C. W. 30 years of campylobacters: biochemical characteristics and a biotyping proposal for Campylobacter jejuni. J Clin Microbiol. 1982 Jun;15(6):1065–1073. doi: 10.1128/jcm.15.6.1065-1073.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jones D. M., Abbott J. D., Painter M. J., Sutcliffe E. M. A comparison of biotypes and serotypes of Campylobacter sp. isolated from patients with enteritis and from animal and environmental sources. J Infect. 1984 Jul;9(1):51–58. doi: 10.1016/s0163-4453(84)94498-0. [DOI] [PubMed] [Google Scholar]
  12. Lior H. New, extended biotyping scheme for Campylobacter jejuni, Campylobacter coli, and "Campylobacter laridis". J Clin Microbiol. 1984 Oct;20(4):636–640. doi: 10.1128/jcm.20.4.636-640.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lior H., Woodward D. L., Edgar J. A., Laroche L. J., Gill P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol. 1982 May;15(5):761–768. doi: 10.1128/jcm.15.5.761-768.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Penner J. L., Hennessy J. N. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol. 1980 Dec;12(6):732–737. doi: 10.1128/jcm.12.6.732-737.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Riley L. W., Finch M. J. Results of the first year of national surveillance of Campylobacter infections in the United States. J Infect Dis. 1985 May;151(5):956–959. doi: 10.1093/infdis/151.5.956. [DOI] [PubMed] [Google Scholar]
  16. Roop R. M., 2nd, Smibert R. M., Johnson J. L., Krieg N. R. Differential characteristics of catalase-positive campylobacters correlated with DNA homology groups. Can J Microbiol. 1984 Jul;30(7):938–951. doi: 10.1139/m84-147. [DOI] [PubMed] [Google Scholar]
  17. Roop R. M., 2nd, Smibert R. M., Krieg N. R. Improved biotyping schemes for Campylobacter jejuni and Campylobacter coli. J Clin Microbiol. 1984 Nov;20(5):990–992. doi: 10.1128/jcm.20.5.990-992.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rosef O., Kapperud G., Lauwers S., Gondrosen B. Serotyping of Campylobacter jejuni, Campylobacter coli, and Campylobacter laridis from domestic and wild animals. Appl Environ Microbiol. 1985 Jun;49(6):1507–1510. doi: 10.1128/aem.49.6.1507-1510.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Selander R. K., Levin B. R. Genetic diversity and structure in Escherichia coli populations. Science. 1980 Oct 31;210(4469):545–547. doi: 10.1126/science.6999623. [DOI] [PubMed] [Google Scholar]
  21. Whittam T. S., Ochman H., Selander R. K. Multilocus genetic structure in natural populations of Escherichia coli. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1751–1755. doi: 10.1073/pnas.80.6.1751. [DOI] [PMC free article] [PubMed] [Google Scholar]

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