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. 1989 May;57(5):1632–1634. doi: 10.1128/iai.57.5.1632-1634.1989

Cathepsin D-like activity in neutrophils and monocytes.

J Levy 1, G B Kolski 1, S D Douglas 1
PMCID: PMC313327  PMID: 2707864

Abstract

Monocytes-macrophages and polymorphonuclear leukocytes contain an acid proteolytic enzyme that cleaves tritiated hemoglobin. The monocyte-macrophage-derived enzymatic activity was completely inhibited by pepstatin A, a property of cathepsin D. Monocyte-derived macrophages developed detectable cathepsin D-like activity after 5 days in culture, and this activity coincided with the appearance of other known indicators of macrophage maturation. The cathepsin D activity further increased significantly with time after day 5 of culture. The proteinase activity extracted from neutrophils was only partially inhibitable by pepstatin A, which indicates that this activity is contributed by more than one proteolytic enzyme, including cathepsin D. Cathepsin D activity demonstrated in neutrophils and macrophages may be an important marker of phagocyte function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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