Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1989 Jun;57(6):1697–1701. doi: 10.1128/iai.57.6.1697-1701.1989

Serotype 8- and serocluster-specific surface-expressed antigens of Ureaplasma urealyticum.

D Thirkell 1, A D Myles 1, W C Russell 1
PMCID: PMC313342  PMID: 2470677

Abstract

The polypeptides of all 14 serotypes of Ureaplasma urealyticum were analyzed by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining. The electrophoretic patterns did not allow ready discrimination of individual serotypes or seroclusters. The analysis of the antigens of serotype 8 was reported previously (B. L. Precious, D. Thirkell, and W. C. Russell, J. Gen. Microbiol. 133:2659-2676, 1987). In this study, three of the surface-expressed membrane antigens of 16, 17, and 96 kilodaltons were further investigated, and monoclonal antibodies were raised against these three polypeptides. The major 96-kilodalton polypeptide was serotype 8 specific, and the 16-kilodalton polypeptide was present only in the larger serocluster. We describe monoclonal antibody probes that unequivocally differentiate serotype 8 from the other serotypes and that separate the two seroclusters of the organism.

Full text

PDF
1697

Images in this article

1699Image
on p.1699
1699Image
on p.1699
1700Image
on p.1700
1700Image
on p.1700

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bordier C. Phase separation of integral membrane proteins in Triton X-114 solution. J Biol Chem. 1981 Feb 25;256(4):1604–1607. [PubMed] [Google Scholar]
  2. Cassell G. H., Waites K. B., Crouse D. T., Rudd P. T., Canupp K. C., Stagno S., Cutter G. R. Association of Ureaplasma urealyticum infection of the lower respiratory tract with chronic lung disease and death in very-low-birth-weight infants. Lancet. 1988 Jul 30;2(8605):240–245. doi: 10.1016/s0140-6736(88)92536-6. [DOI] [PubMed] [Google Scholar]
  3. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Horowitz S. A., Duffy L., Garrett B., Stephens J., Davis J. K., Cassell G. H. Can group- and serovar-specific proteins be detected in Ureaplasma urealyticum? Pediatr Infect Dis. 1986 Nov-Dec;5(6 Suppl):S325–S331. doi: 10.1097/00006454-198611010-00029. [DOI] [PubMed] [Google Scholar]
  5. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  6. Mautner V., Willcox H. N. Adenovirus antigens: a model system in mice for subunit vaccination. J Gen Virol. 1974 Dec;25(3):325–336. doi: 10.1099/0022-1317-25-3-325. [DOI] [PubMed] [Google Scholar]
  7. Mouches C., Taylor-Robinson D., Stipkovits L., Bove J. M. Comparison of human and animal Ureaplasmas by one- and two-dimensional protein analysis on polyacrylamide slab gel. Ann Microbiol (Paris) 1981 Sep-Oct;132B(2):171–196. [PubMed] [Google Scholar]
  8. Precious B. L., Thirkell D., Russell W. C. Preliminary characterization of the urease and a 96 kDa surface-expressed polypeptide of Ureaplasma urealyticum. J Gen Microbiol. 1987 Sep;133(9):2659–2670. doi: 10.1099/00221287-133-9-2659. [DOI] [PubMed] [Google Scholar]
  9. Randall R. E., Newman C., Honess R. W. Isolation and characterization of monoclonal antibodies to structural and nonstructural herpesvirus saimiri proteins. J Virol. 1984 Dec;52(3):872–883. doi: 10.1128/jvi.52.3.872-883.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Riethman H. C., Boyer M. J., Wise K. S. Triton X-114 phase fractionation of an integral membrane surface protein mediating monoclonal antibody killing of Mycoplasma hyorhinis. Infect Immun. 1987 May;55(5):1094–1100. doi: 10.1128/iai.55.5.1094-1100.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Robertson J. A., Chen M. H. Effects of manganese on the growth and morphology of Ureaplasma urealyticum. J Clin Microbiol. 1984 Jun;19(6):857–864. doi: 10.1128/jcm.19.6.857-864.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robertson J. A., Honore L. H., Stemke G. W. Serotypes of Ureaplasma urealyticum in spontaneous abortion. Pediatr Infect Dis. 1986 Nov-Dec;5(6 Suppl):S270–S272. doi: 10.1097/00006454-198611010-00014. [DOI] [PubMed] [Google Scholar]
  13. Robertson J. A. Potential virulence factors of Ureaplasma urealyticum. Pediatr Infect Dis. 1986 Nov-Dec;5(6 Suppl):S322–S324. doi: 10.1097/00006454-198611010-00028. [DOI] [PubMed] [Google Scholar]
  14. Robertson J. A., Stemke G. W. Expanded serotyping scheme for Ureaplasma urealyticum strains isolated from humans. J Clin Microbiol. 1982 May;15(5):873–878. doi: 10.1128/jcm.15.5.873-878.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Russell W. C., Blair G. E. Polypeptide phosphorylation in adenovirus-infected cells. J Gen Virol. 1977 Jan;34(1):19–35. doi: 10.1099/0022-1317-34-1-19. [DOI] [PubMed] [Google Scholar]
  16. Russell W. C., Patel G., Precious B., Sharp I., Gardner P. S. Monoclonal antibodies against adenovirus type 5: preparation and preliminary characterization. J Gen Virol. 1981 Oct;56(Pt 2):393–408. doi: 10.1099/0022-1317-56-2-393. [DOI] [PubMed] [Google Scholar]
  17. Stemke G. W., Robertson J. A. Problems associated with serotyping strains of Ureaplasma urealyticum. Diagn Microbiol Infect Dis. 1985 Jul;3(4):311–320. doi: 10.1016/0732-8893(85)90005-7. [DOI] [PubMed] [Google Scholar]
  18. Thirkell D., Myles A. D., Precious B. L., Frost J. S., Woodall J. C., Burdon M. G., Russell W. C. The urease of Ureaplasma urealyticum. J Gen Microbiol. 1989 Feb;135(Pt 2):315–323. doi: 10.1099/00221287-135-2-315. [DOI] [PubMed] [Google Scholar]
  19. Waites K. B., Rudd P. T., Crouse D. T., Canupp K. C., Nelson K. G., Ramsey C., Cassell G. H. Chronic Ureaplasma urealyticum and Mycoplasma hominis infections of central nervous system in preterm infants. Lancet. 1988 Jan 2;1(8575-6):17–21. doi: 10.1016/s0140-6736(88)91002-1. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES