Table 1.

Comparison of msp2 and msp3 genes between pyrosequenced and Sanger-sequenced Anaplasma genomes. Genes detected as present (+) or absent (−) by pyrosequencing are shown together with their % sequence identity (of the best match, by MATGAT), in parentheses. The respective gene annotations are shown at the top. All genes with <90% sequence identity between strains were detected as absent. Mean genome coverage for pyrosequencing data is indicated in the left column. Genome abbreviations: FL – Florida, StM – St. Maries, ACIS – A. marginale subspecies centrale, Israel.

	FL msp2								StM msp2							ACIS msp2
Pyro
Annot.	25	47	155	534	757	872	945	1018	33	49	213	720	1152	1250	1344	29	47	50	145	601	1176	1183
FL	(100)	(100)	(100)	(100)	(100)	(100)	(100)	(100)	(99)	(92)	(78)	(92)	(100)	(100)	(100)	(79)	(76)	(79)	(75)	(81)	(79)	(77)
59X	+	+	+	+	+	+	+	+	+	+	−	+	+	+	+	−	−	−	−	−	−	−
FL-rel
76X	+	+	+	+	+	+	+	+	+	+	−	+	+	+	+	−	−	−	−	−	−	−
StM	(99)	(92)	(90)	(92)	(100)	(100)	(100)	(91)	(100)	(100)	(100)	(100)	(100)	(100)	(100)	(78)	(74)	(78)	(73)	(80)	(79)	(77)
117X	+	+	−	+	+	+	+	−	+	+	+	+	+	+	+	−	−	−	−	−	−	−

	FL msp3							StM msp3							ACIS msp3
Pyro
Annot.	34	58	65	533	871	1019	1097	47	62	78	91	718	1150	1345	70	144	298	354	393	1076	1173	1180
FL	(100)	(100)	(100)	(100)	(100)	(100)	(100)	(100)	(60)	(57)	(76)	(100)	(97)	(78)	(58)	(58)	(59)	(54)	(57)	(52)	(60)	(59)
59X	+	+	+	+	+	+	+	+	−	−	−	+	+	−	−	−	−	−	−	−	−	−
FL-rel
76X	+	+	+	+	+	+	+	+	−	−	−	+	+	−	−	−	−	−	−	−	−	−
StM	(59)	(56)	(68)	(100)	(97)	(55)	(100)	(100)	(100)	(100)	(100)	(100)	(100)	(100)	(59)	(58)	(59)	(53)	(61)	(52)	(60)	(59)
117X	−	−	−	+	+	−	+	+	+	+	+	+	+	+	−	−	−	−	−	−	−	−