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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Biomaterials. 2011 Jun 11;32(27):6412–6424. doi: 10.1016/j.biomaterials.2011.05.034

Figure 7. Inhibition of cell attachment.

Figure 7

Inhibition of hMSCs attachment with soluble CLP-Cys peptide and anti-α2β1 integrin antibody on Glass-CLP-Cys and HA-(HGP-CLP-Cys). Suspended cells were preincubated with different concentrations of the CLP-Cys peptide or anti-integrin α2β1 antibody (50 µg/mL) for 30 minutes at 37 °C and then allowed to attach to the Glass-CLP-Cys or HA-(HGP-CLP-Cys). The number of hMSCs attached to these surfaces, when the hMSCs were not preincubated with either the soluble CLP-Cys or anti-α2β1 integrin antibody was taken as 100 percent attachment. The fraction of cells attached was calculated relative to the fraction of cells that attached to the control surface. * and # are statistically different from each other (Student’s t test with p < 0.05). Each histogram represents the mean ± standard deviation of three repeats.