Table 1.

CD4⁺CD25⁺:Treg ratio in coculture of CD4⁺ T cells plus autologous DCs treated with yeast or with CD40L (HD#1)

Coculture (ratio)	Total number of cells/well	CD4+ (% in total number of cells)	CD4+CD25+ (% in CD4+)	Tregs		Ratio effector cells:Tregs
				(% in CD4+)	(% in CD4+CD25+)
CD4+ : CD40L- treated DCs (10:1)	2.65 × 106	2.41 × 106 (91.2%)	2.12 × 105 (8.8%)	0.86 × 105 (3.5%)	40.3%	1.5:1
CD4+ : YEAST- treated DCs (10:1)	3.50 × 106	3.10 × 106 (88.8%)	3.59 × 105 (11.6%)	1.10 × 105 (4.4%)	30.6%	2.4:1
CD4+ : CD40L- treated DCs (5:1)	2.81 × 106	2.50 × 106 (89.3%)	2.12 × 105 (8.5%)	1.10 × 105 (3.4%)	51.8%	0.9:1
CD4+ : YEAST- treated DCs (5:1)	4.12 × 106	3.53 × 106 (85.7%)	6.81 × 105 (19.3%)	1.41 × 105 (4.0%)	20.7%	3.8:1

Tregs = CD4⁺CD25⁺CD127⁻FoxP3⁺

Effector cells = CD4⁺CD25⁺

DCs were treated with yeast at a yeast:DC ratio of 10:1 for 48 h or with CD40L (1 μg/ml) for 24 h. Isolated autologous CD4⁺ T cells were cocultured with yeast-treated DCs or CD40L-treated DCs at 2 ratios (10:1 or 5:1). FACS analysis was performed on day 5.

Ratio of effector cells:Tregs = ([CD4+CD25+] - [CD4+CD25+CD127-FoxP3+Tregs]/[CD4+CD25+CD127-FoxP3+Tregs]).

HD = healthy donor.