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. 1989 Aug;57(8):2272–2278. doi: 10.1128/iai.57.8.2272-2278.1989

Receptor-mediated adherence of Campylobacter pylori to mouse Y-1 adrenal cell monolayers.

D G Evans 1, D J Evans Jr 1, D Y Graham 1
PMCID: PMC313441  PMID: 2473033

Abstract

An in vitro adherence assay was developed to study the adherence of Campylobacter pylori to mammalian cells. Strains of C. pylori were isolated from individuals with gastritis. These strains possessed the fibrillar N-acetylneuraminyllactose(neuraminlactose)-binding hemagglutinin (NLBH) and were found to adhere to monolayers of mouse Y-1 adrenal cells. Adherence was rapid, prevented by pretreatment of the Y-1 cells with Clostridium perfringens neuraminidase, and blocked by the neuraminlactose-containing protein fetuin. Electron microscopy by the immunogold technique and with anti-NLBH antibody showed that NLBH was present at the sites of interaction between C. pylori and the Y-1 cells. These results indicate that the Y-1 monolayer is a valid model for receptor-specific adherence of C. pylori.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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