Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1989 Aug;57(8):2313–2317. doi: 10.1128/iai.57.8.2313-2317.1989

Modulation of colonization by black-pigmented Bacteroides species in squirrel monkeys by immunization with Bacteroides gingivalis.

W P McArthur 1, I Magnusson 1, R G Marks 1, W B Clark 1
PMCID: PMC313448  PMID: 2744849

Abstract

Periodontal diseases are inflammatory responses thought to be triggered by specific microorganisms colonizing in the gingival crevice. Theoretically, periodontal diseases could be prevented if the etiologic organisms were not allowed to colonize the subgingival area. The humoral immune response is one mechanism which may modulate bacterial colonization in the gingival crevice. To test the effect of systemic humoral immunity on subgingival colonization by bacteria, squirrel monkeys (Saimiri sciureus) were immunized with Bacteroides gingivalis, a black-pigmented Bacteroides sp. and putative periodontal pathogen. Immunized and sham-immunized monkeys were orally inoculated with 10(10) viable B. gingivalis during ligation of five teeth in one quadrant with bacterium-soaked suture material and distribution over the entire dentogingival margin. Immunization resulted in an increased level of immunoglobulin G anti-B. gingivalis in serum and was associated with a strong trend toward a statistically significant reduction in colonization of the gingival crevice by black-pigmented bacteroides.

Full text

PDF
2313

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams R. A., Zander H. A., Polson A. M. Interproximal and buccal cell populations apical to the sulcus before and during experimental periodontitis in squirrel monkeys. J Periodontol. 1981 Aug;52(8):416–419. doi: 10.1902/jop.1981.52.8.416. [DOI] [PubMed] [Google Scholar]
  2. Attström R., Laurel A. B., Lahsson U., Sjöholm A. Complement factors in gingival crevice material from healthy and inflamed gingiva in humans. J Periodontal Res. 1975 Feb;10(1):19–27. doi: 10.1111/j.1600-0765.1975.tb00003.x. [DOI] [PubMed] [Google Scholar]
  3. BAILIT H. L., BALDWIN D. C., HUNT E. E., Jr THE INCREASING PREVALENCE OF GINGIVAL BACTEROIDES MELANINOGENICUS WITH AGE IN CHILDREN. Arch Oral Biol. 1964 Jul-Aug;9:435–438. doi: 10.1016/0003-9969(64)90028-7. [DOI] [PubMed] [Google Scholar]
  4. Bammann L. L., Clark W. B., Gibbons R. J. Impaired colonization of gnotobiotic and conventional rats by streptomycin-resistant strains of Streptococcus mutans. Infect Immun. 1978 Dec;22(3):721–726. doi: 10.1128/iai.22.3.721-726.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clark W. B., Magnusson I., Abee C., Collins B., Beem J. E., McArthur W. P. Natural occurrence of black-pigmented Bacteroides species in the gingival crevice of the squirrel monkey. Infect Immun. 1988 Sep;56(9):2392–2399. doi: 10.1128/iai.56.9.2392-2399.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gold O. G., Jordan H. V., Van Houte J. A selective medium for Streptococcus mutans. Arch Oral Biol. 1973 Nov;18(11):1357–1364. doi: 10.1016/0003-9969(73)90109-x. [DOI] [PubMed] [Google Scholar]
  7. Heijl L., Rifkin B. R., Zander H. A. Conversion of chronic gingivitis to periodontitis in squirrel monkeys. J Periodontol. 1976 Dec;47(12):710–716. doi: 10.1902/jop.1976.47.12.710. [DOI] [PubMed] [Google Scholar]
  8. Holt S. C., Ebersole J., Felton J., Brunsvold M., Kornman K. S. Implantation of Bacteroides gingivalis in nonhuman primates initiates progression of periodontitis. Science. 1988 Jan 1;239(4835):55–57. doi: 10.1126/science.3336774. [DOI] [PubMed] [Google Scholar]
  9. Kennedy J. E., Polson A. M. Experimental marginal periodontitis in squirrel monkeys. J Periodontol. 1973 Mar;44(3):140–144. doi: 10.1902/jop.1973.44.3.140. [DOI] [PubMed] [Google Scholar]
  10. Lehner T., Challacombe S. J., Caldwell J. Oral immunization with Streptococcus mutants in rhesus monkeys and the development of immune response and dental caries. Immunology. 1980 Dec;41(4):857–864. [PMC free article] [PubMed] [Google Scholar]
  11. McArthur W. P., Stroup S., Wilson L. Detection and serotyping of Actinobacillus actinomycetemcomitans isolates on nitrocellulose paper blots with monoclonal antibodies. J Clin Periodontol. 1986 Aug;13(7):684–691. doi: 10.1111/j.1600-051x.1986.tb00866.x. [DOI] [PubMed] [Google Scholar]
  12. Mouton C., Hammond P. G., Slots J., Genco R. J. Serum antibodies to oral Bacteroides asaccharolyticus (Bacteroides gingivalis): relationship to age and periondontal disease. Infect Immun. 1981 Jan;31(1):182–192. doi: 10.1128/iai.31.1.182-192.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Okuda K., Kato T., Naito Y., Takazoe I., Kikuchi Y., Nakamura T., Kiyoshige T., Sasaki S. Protective efficacy of active and passive immunizations against experimental infection with Bacteroides gingivalis in ligated hamsters. J Dent Res. 1988 May;67(5):807–811. doi: 10.1177/00220345880670050201. [DOI] [PubMed] [Google Scholar]
  14. Slots J., Emrich L. J., Genco R. J., Rosling B. G. Relationship between some subgingival bacteria and periodontal pocket depth and gain or loss of periodontal attachment after treatment of adult periodontitis. J Clin Periodontol. 1985 Aug;12(7):540–552. doi: 10.1111/j.1600-051x.1985.tb01388.x. [DOI] [PubMed] [Google Scholar]
  15. Slots J. Selective medium for isolation of Actinobacillus actinomycetemcomitans. J Clin Microbiol. 1982 Apr;15(4):606–609. doi: 10.1128/jcm.15.4.606-609.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Slots J. Subgingival microflora and periodontal disease. J Clin Periodontol. 1979 Oct;6(5):351–382. doi: 10.1111/j.1600-051x.1979.tb01935.x. [DOI] [PubMed] [Google Scholar]
  17. Syed S. A., Svanberg M., Svanberg G. The predominant cultivable dental plaque flora of beagle dogs with gingivitis. J Periodontal Res. 1980 Mar;15(2):123–136. doi: 10.1111/j.1600-0765.1980.tb00266.x. [DOI] [PubMed] [Google Scholar]
  18. Walker C. B., Ratliff D., Muller D., Mandell R., Socransky S. S. Medium for selective isolation of Fusobacterium nucleatum from human periodontal pockets. J Clin Microbiol. 1979 Dec;10(6):844–849. doi: 10.1128/jcm.10.6.844-849.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. White D., Mayrand D. Association of oral Bacteroides with gingivitis and adult periodontitis. J Periodontal Res. 1981 May;16(3):259–265. doi: 10.1111/j.1600-0765.1981.tb00974.x. [DOI] [PubMed] [Google Scholar]
  20. Zylber L. J., Jordan H. V. Development of a selective medium for detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque. J Clin Microbiol. 1982 Feb;15(2):253–259. doi: 10.1128/jcm.15.2.253-259.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES