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. 1989 Aug;57(8):2588–2590. doi: 10.1128/iai.57.8.2588-2590.1989

Cloning of a hemolysin gene from Leptospira interrogans serovar hardjo.

G del Real 1, R P Segers 1, B A van der Zeijst 1, W Gaastra 1
PMCID: PMC313494  PMID: 2744864

Abstract

A DNA fragment encoding both hemolysin and sphingomyelinase C activity was cloned from the pathogenic bacterium Leptospira interrogans serovar hardjo. Initial clones were obtained by screening a genomic library in EMBL3 for hemolytic activity. Both hemolytic and sphingomyelinase C activities were coded for by a 3.9-kilobase BamHI fragment. The hemolysin was expressed from its own promoter in Escherichia coli K-12. Similar DNA sequences were also present in the serovars tarassovi and ballum.

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Selected References

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