Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1989 Sep;57(9):2903–2905. doi: 10.1128/iai.57.9.2903-2905.1989

Macrophage cell lines P388D1 and IC-21 stimulated with gamma interferon fail to inhibit the intracellular growth of Histoplasma capsulatum.

B Wu-Hsieh 1, D H Howard 1
PMCID: PMC313545  PMID: 2503448

Abstract

Histoplasma capsulatum, a facultative intracellular parasite of macrophages, grows within mononuclear cells of the P388D1 and IC-21 cell lines with a generation time comparable to that with which it grows in normal resident peritoneal macrophages (10 +/- 2 h). Recombinant murine gamma interferon (rMuIFN-gamma) activates P388D1 cells to express la antigens but not to inhibit the intracellular growth of H. capsulatum, alone or in combination with lipopolysaccharide. IC-21 cells also could not be activated to fungistasis with rMuIFN-gamma. Explanted resident peritoneal macrophages of the C57BL/6 (from which the IC-21 cell line derives), C3H/HeJ, DBA/2 (from which the P388D1 cell line derives), A/J, and SJL/J strains of mice were all stimulated by rMuIFN-gamma to inhibit the fungus.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams D. O., Hamilton T. A. Molecular transductional mechanisms by which IFN gamma and other signals regulate macrophage development. Immunol Rev. 1987 Jun;97:5–27. doi: 10.1111/j.1600-065x.1987.tb00514.x. [DOI] [PubMed] [Google Scholar]
  2. Adams D. O., Hamilton T. A. The cell biology of macrophage activation. Annu Rev Immunol. 1984;2:283–318. doi: 10.1146/annurev.iy.02.040184.001435. [DOI] [PubMed] [Google Scholar]
  3. Blumenthal E. J., Roberts W. K., Vasil A., Talmage D. W. Macrophage activation: dissociation of cytotoxic activity from Ia-A antigen expression. Proc Natl Acad Sci U S A. 1983 Apr;80(7):2031–2035. doi: 10.1073/pnas.80.7.2031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chick E. W., Roberts G. D. The varying susceptibility of different genetic strains of laboratory mice to Histoplasma capsulatum. Mycopathol Mycol Appl. 1974 Apr 30;52(3):251–253. doi: 10.1007/BF02198750. [DOI] [PubMed] [Google Scholar]
  5. Eissenberg L. G., Schlesinger P. H., Goldman W. E. Phagosome-lysosome fusion in P388D1 macrophages infected with Histoplasma capsulatum. J Leukoc Biol. 1988 Jun;43(6):483–491. doi: 10.1002/jlb.43.6.483. [DOI] [PubMed] [Google Scholar]
  6. Howard D. H., Otto V., Gupta R. K. Lymphocyte-mediated cellular immunity in histoplasmosis. Infect Immun. 1971 Nov;4(5):605–610. doi: 10.1128/iai.4.5.605-610.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mauel J., Defendi V. Infection and transformation of mouse peritoneal macrophages by simian virus 40. J Exp Med. 1971 Aug 1;134(2):335–350. doi: 10.1084/jem.134.2.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moon D. K., Geczy C. L. Recombinant IFN-gamma synergizes with lipopolysaccharide to induce macrophage membrane procoagulants. J Immunol. 1988 Sep 1;141(5):1536–1542. [PubMed] [Google Scholar]
  9. Wu-Hsieh B. A., Howard D. H. Inhibition of the intracellular growth of Histoplasma capsulatum by recombinant murine gamma interferon. Infect Immun. 1987 Apr;55(4):1014–1016. doi: 10.1128/iai.55.4.1014-1016.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wu-Hsieh B., Howard D. H. Inhibition of growth of Histoplasma capsulatum by lymphokine-stimulated macrophages. J Immunol. 1984 May;132(5):2593–2597. [PubMed] [Google Scholar]
  11. Wu-Hsieh B., Zlotnik A., Howard D. H. T-cell hybridoma-produced lymphokine that activates macrophages to suppress intracellular growth of Histoplasma capsulatum. Infect Immun. 1984 Jan;43(1):380–385. doi: 10.1128/iai.43.1.380-385.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES