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. 1990 Sep;58(9):2957–2961. doi: 10.1128/iai.58.9.2957-2961.1990

Neither motility nor chemotaxis plays a role in the ability of Escherichia coli F-18 to colonize the streptomycin-treated mouse large intestine.

B A McCormick 1, D C Laux 1, P S Cohen 1
PMCID: PMC313594  PMID: 2201640

Abstract

Escherichia coli F-18, isolated from the feces of a healthy human in 1977, is an excellent colonizer of the streptomycin-treated mouse large intestine and displays normal motility and chemotaxis ability. A chemotaxis-defective derivative of E. coli F-18, E, coli F-18 CheA-, and a nonflagellated derivative, E. coli F-18 Fla-, were constructed. These strains were found to colonize the streptomycin-treated mouse large intestine as well as E. coli F-18 when mice were fed both E. coli F-18 and either the CheA- or Fla- derivative at high levels (10(10) CFU of each strain per mouse) or low levels (10(4) CFU of each strain per mouse). Furthermore, E. coli F-18 lost motility and chemotaxis ability when grown in colonic or cecal mucus in vitro despite retaining the ability to synthesize flagella. Thus, it appears that neither motility nor chemotaxis plays a role in the ability of E. coli F-18 to colonize because this strain becomes functionally nonmotile upon growth in the streptomycin-treated mouse large intestine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arthur M., Johnson C. E., Rubin R. H., Arbeit R. D., Campanelli C., Kim C., Steinbach S., Agarwal M., Wilkinson R., Goldstein R. Molecular epidemiology of adhesin and hemolysin virulence factors among uropathogenic Escherichia coli. Infect Immun. 1989 Feb;57(2):303–313. doi: 10.1128/iai.57.2.303-313.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOHNHOFF M., MILLER C. P. Enhanced susceptibility to Salmonella infection in streptomycin-treated mice. J Infect Dis. 1962 Sep-Oct;111:117–127. doi: 10.1093/infdis/111.2.117. [DOI] [PubMed] [Google Scholar]
  3. Caldwell M. B., Guerry P., Lee E. C., Burans J. P., Walker R. I. Reversible expression of flagella in Campylobacter jejuni. Infect Immun. 1985 Dec;50(3):941–943. doi: 10.1128/iai.50.3.941-943.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carsiotis M., Stocker B. A., Weinstein D. L., O'Brien A. D. A Salmonella typhimurium virulence gene linked to flg. Infect Immun. 1989 Nov;57(11):3276–3280. doi: 10.1128/iai.57.11.3276-3280.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen P. S., Kjelleberg S., Laux D. C., Conway P. L. Escherichia coli F-18 makes a streptomycin-treated mouse large intestine colonization factor when grown in nutrient broth containing glucose. Infect Immun. 1990 May;58(5):1471–1472. doi: 10.1128/iai.58.5.1471-1472.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen P. S., Rossoll R., Cabelli V. J., Yang S. L., Laux D. C. Relationship between the mouse colonizing ability of a human fecal Escherichia coli strain and its ability to bind a specific mouse colonic mucous gel protein. Infect Immun. 1983 Apr;40(1):62–69. doi: 10.1128/iai.40.1.62-69.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FRETER R. Experimental enteric Shigella and Vibrio infections in mice and guinea pigs. J Exp Med. 1956 Sep 1;104(3):411–418. doi: 10.1084/jem.104.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Freter R., O'Brien P. C., Macsai M. S. Role of chemotaxis in the association of motile bacteria with intestinal mucosa: in vivo studies. Infect Immun. 1981 Oct;34(1):234–240. doi: 10.1128/iai.34.1.234-240.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Komeda Y., Icho T., Iino T. Effects of galU mutation on flagellar formation in Escherichia coli. J Bacteriol. 1977 Feb;129(2):908–915. doi: 10.1128/jb.129.2.908-915.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lockman H. A., Curtiss R., 3rd Salmonella typhimurium mutants lacking flagella or motility remain virulent in BALB/c mice. Infect Immun. 1990 Jan;58(1):137–143. doi: 10.1128/iai.58.1.137-143.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McCormick B. A., Franklin D. P., Laux D. C., Cohen P. S. Type 1 pili are not necessary for colonization of the streptomycin-treated mouse large intestine by type 1-piliated Escherichia coli F-18 and E. coli K-12. Infect Immun. 1989 Oct;57(10):3022–3029. doi: 10.1128/iai.57.10.3022-3029.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McCormick B. A., Stocker B. A., Laux D. C., Cohen P. S. Roles of motility, chemotaxis, and penetration through and growth in intestinal mucus in the ability of an avirulent strain of Salmonella typhimurium to colonize the large intestine of streptomycin-treated mice. Infect Immun. 1988 Sep;56(9):2209–2217. doi: 10.1128/iai.56.9.2209-2217.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morooka T., Umeda A., Amako K. Motility as an intestinal colonization factor for Campylobacter jejuni. J Gen Microbiol. 1985 Aug;131(8):1973–1980. doi: 10.1099/00221287-131-8-1973. [DOI] [PubMed] [Google Scholar]
  14. Nevola J. J., Stocker B. A., Laux D. C., Cohen P. S. Colonization of the mouse intestine by an avirulent Salmonella typhimurium strain and its lipopolysaccharide-defective mutants. Infect Immun. 1985 Oct;50(1):152–159. doi: 10.1128/iai.50.1.152-159.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Revel H. R. Restriction of nonglucosylated T-even bacteriophage: properties of permissive mutants of Escherichia coli B and K12. Virology. 1967 Apr;31(4):688–701. doi: 10.1016/0042-6822(67)90197-3. [DOI] [PubMed] [Google Scholar]
  16. Stanton T. B., Savage D. C. Motility as a factor in bowel colonization by Roseburia cecicola, an obligately anaerobic bacterium from the mouse caecum. J Gen Microbiol. 1984 Jan;130(1):173–183. doi: 10.1099/00221287-130-1-173. [DOI] [PubMed] [Google Scholar]
  17. Wadolkowski E. A., Laux D. C., Cohen P. S. Colonization of the streptomycin-treated mouse large intestine by a human fecal Escherichia coli strain: role of growth in mucus. Infect Immun. 1988 May;56(5):1030–1035. doi: 10.1128/iai.56.5.1030-1035.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. van der Waaij D., Berghuis J. M., Lekkerkerk J. E. Colonization resistance of the digestive tract of mice during systemic antibiotic treatment. J Hyg (Lond) 1972 Dec;70(4):605–610. doi: 10.1017/s0022172400022464. [DOI] [PMC free article] [PubMed] [Google Scholar]

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