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. 1990 Sep;58(9):3064–3072. doi: 10.1128/iai.58.9.3064-3072.1990

Characterization of Streptococcus gordonii (S. sanguis) PK488 adhesin-mediated coaggregation with Actinomyces naeslundii PK606.

P E Kolenbrander 1, R N Andersen 1
PMCID: PMC313612  PMID: 2387635

Abstract

Intergeneric coaggregation of Streptococcus gordonii (S. sanguis) PK488 and Actinomyces naeslundii PK606 was studied by using coaggregation-defective (Cog-) mutants of both strains. A streptococcal protein of 38 kilodaltons was identified with anti-S. gordonii serum absorbed with Cog- cells of the streptococcus. Absorbed immunoglobulin G specifically blocked coaggregation of the streptococcus-actinomyces pair but did not affect the coaggregation of the streptococcus with other coaggregation partners. The 38-kilodalton protein was found in the supernatant of mild sonicated cell suspensions and was extracted from whole cells with sodium barbital or with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS). An immunoreactive protein of the same size was found in sonicated cell supernatants of several other oral streptococci that also coaggregated with A. naeslundii PK606. Inhibition of S. gordonii PK488-A. naeslundii PK606 coaggregation was not observed with any of 16 different sugars tested. We propose that a functionally similar adhesin that mediates coaggregations with A. naeslundii PK606 is expressed by several species of the genus Streptococcus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abeygunawardana C., Bush C. A., Cisar J. O. Complete structure of the polysaccharide from Streptococcus sanguis J22. Biochemistry. 1990 Jan 9;29(1):234–248. doi: 10.1021/bi00453a032. [DOI] [PubMed] [Google Scholar]
  2. Batteiger B., Newhall W. J., 5th, Jones R. B. The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J Immunol Methods. 1982 Dec 30;55(3):297–307. doi: 10.1016/0022-1759(82)90089-8. [DOI] [PubMed] [Google Scholar]
  3. Cisar J. O., Kolenbrander P. E., McIntire F. C. Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii. Infect Immun. 1979 Jun;24(3):742–752. doi: 10.1128/iai.24.3.742-752.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. King J., Laemmli U. K. Polypeptides of the tail fibres of bacteriophage T4. J Mol Biol. 1971 Dec 28;62(3):465–477. doi: 10.1016/0022-2836(71)90148-3. [DOI] [PubMed] [Google Scholar]
  5. Kolenbrander P. E., Andersen R. N. Multigeneric aggregations among oral bacteria: a network of independent cell-to-cell interactions. J Bacteriol. 1986 Nov;168(2):851–859. doi: 10.1128/jb.168.2.851-859.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kolenbrander P. E., Inouye Y., Holdeman L. V. New Actinomyces and Streptococcus coaggregation groups among human oral isolates from the same site. Infect Immun. 1983 Aug;41(2):501–506. doi: 10.1128/iai.41.2.501-506.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kolenbrander P. E. Intergeneric coaggregation among human oral bacteria and ecology of dental plaque. Annu Rev Microbiol. 1988;42:627–656. doi: 10.1146/annurev.mi.42.100188.003211. [DOI] [PubMed] [Google Scholar]
  8. Kolenbrander P. E. Isolation and characterization of coaggregation-defective mutants of Actinomyces viscosus, Actinomyces naeslundii, and Streptococcus sanguis. Infect Immun. 1982 Sep;37(3):1200–1208. doi: 10.1128/iai.37.3.1200-1208.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kolenbrander P. E. Surface recognition among oral bacteria: multigeneric coaggregations and their mediators. Crit Rev Microbiol. 1989;17(2):137–159. doi: 10.3109/10408418909105746. [DOI] [PubMed] [Google Scholar]
  10. Kolenbrander P. E., Williams B. L. Lactose-reversible coaggregation between oral actinomycetes and Streptococcus sanguis. Infect Immun. 1981 Jul;33(1):95–102. doi: 10.1128/iai.33.1.95-102.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kolenbrander P. E., Williams B. L. Prevalence of viridans streptococci exhibiting lactose-inhibitable coaggregation with oral actinomycetes. Infect Immun. 1983 Aug;41(2):449–452. doi: 10.1128/iai.41.2.449-452.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lamont R. J., Rosan B., Murphy G. M., Baker C. T. Streptococcus sanguis surface antigens and their interactions with saliva. Infect Immun. 1988 Jan;56(1):64–70. doi: 10.1128/iai.56.1.64-70.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. London J., Allen J. Purification and characterization of a Bacteroides loeschei adhesin that interacts with procaryotic and eucaryotic cells. J Bacteriol. 1990 May;172(5):2527–2534. doi: 10.1128/jb.172.5.2527-2534.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maryanski J. H., Wittenberger C. L. Mannitol transport in Streptococcus mutans. J Bacteriol. 1975 Dec;124(3):1475–1481. doi: 10.1128/jb.124.3.1475-1481.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McIntire F. C., Crosby L. K., Vatter A. E., Cisar J. O., McNeil M. R., Bush C. A., Tjoa S. S., Fennessey P. V. A polysaccharide from Streptococcus sanguis 34 that inhibits coaggregation of S. sanguis 34 with Actinomyces viscosus T14V. J Bacteriol. 1988 May;170(5):2229–2235. doi: 10.1128/jb.170.5.2229-2235.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weiss E. I., Kolenbrander P. E., London J., Hand A. R., Andersen R. N. Fimbria-associated proteins of Bacteroides loescheii PK1295 mediate intergeneric coaggregations. J Bacteriol. 1987 Sep;169(9):4215–4222. doi: 10.1128/jb.169.9.4215-4222.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weiss E. I., London J., Kolenbrander P. E., Andersen R. N., Fischler C., Siraganian R. P. Characterization of monoclonal antibodies to fimbria-associated adhesins of Bacteroides loescheii PK1295. Infect Immun. 1988 Jan;56(1):219–224. doi: 10.1128/iai.56.1.219-224.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weiss E. I., London J., Kolenbrander P. E., Hand A. R., Siraganian R. Localization and enumeration of fimbria-associated adhesins of Bacteroides loescheii. J Bacteriol. 1988 Mar;170(3):1123–1128. doi: 10.1128/jb.170.3.1123-1128.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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