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. 1990 Oct;58(10):3401–3406. doi: 10.1128/iai.58.10.3401-3406.1990

Biochemical and histologic findings in experimental pyelonephritis due to Ureaplasma urealyticum.

W J Pickering 1, D F Birch 1, P Kincaid-Smith 1
PMCID: PMC313667  PMID: 2205581

Abstract

Ureaplasma urealyticum has previously been shown to be capable of persisting in the rat kidney for up to 6 months following a single reflux challenge. We examined kidney tissue from infected animals for evidence of renal damage by using standard cytochemical and immunoenzyme methods. We also monitored changes in renal function during a 6-month study period with standard biochemical assays of plasma and urine. Histologic examination showed tubular atrophy, interstitial fibrosis, and a mononuclear infiltrate in proportion to ureaplasma counts from renal tissue. The most severe damage was accompanied by hyaline cast formation within tubules which gave rise to the typical thyroidlike appearance of chronic pyelonephritis involving conventional urinary pathogens. Macroscopic renal scarring occurred in some animals. Although damage to the renal medulla was moderate to severe, only minor changes were seen in the cortex, and glomeruli were invariably spared. Biochemical tests of renal function showed similar changes in infected and uninfected animals during the study period. Interstitial inflammation was characterized by a mononuclear cell infiltrate in which polymorphonuclear leukocytes were not conspicuous. It is evident that U. urealyticum is capable of producing chronic pyelonephritis in the rat after a single reflux challenge. The results of this study have obvious implications for the pathogenicity of these bacteria in human pyelonephritis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSEN B. R., JACKSON G. G. Pyelitis, an important factor in the pathogenesis of retrograde pyelonephritis. J Exp Med. 1961 Sep 1;114:375–384. doi: 10.1084/jem.114.3.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barclay A. N. The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593–600. [PMC free article] [PubMed] [Google Scholar]
  3. Billett E. E., Gunn B., Mayer R. J. Characterization of two monoclonal antibodies obtained after immunization with human liver mitochondrial membrane preparations. Biochem J. 1984 Aug 1;221(3):765–776. doi: 10.1042/bj2210765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Birch D. F., Fairley K. F., Pavillard R. E. Unconventional bacteria in urinary tract disease: Ureaplasma urealyticum. Kidney Int. 1981 Jan;19(1):58–64. doi: 10.1038/ki.1981.7. [DOI] [PubMed] [Google Scholar]
  5. Birch D. F., Pavillard E. R. Renal infection in the rat after reflux challenge with Pseudomonas aeruginosa. Pathology. 1978 Jul;10(3):269–275. doi: 10.3109/00313027809063511. [DOI] [PubMed] [Google Scholar]
  6. COTRAN R. S. Retrograde Proteus pyelonephritis in rats. Localization of antigen and antibody in treated sterile pyelonephritic kidneys. J Exp Med. 1963 May 1;117:813–822. doi: 10.1084/jem.117.5.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. COTRAN R. S., THRUPP L. D., HAJJ S. N., ZANGWILL D. P., VIVALDI E., KASS E. H. Retrograde E. coli pyelonephritis in the rat: a bacteriologic, pathologic, and fluorescent antibody study. J Lab Clin Med. 1963 Jun;61:987–1004. [PubMed] [Google Scholar]
  8. Fierer J., Talner L., Braude A. I. Bacteremia in the pathogenesis of retrograde E. coli pyelonephritis in the rat. Am J Pathol. 1971 Aug;64(2):443–456. [PMC free article] [PubMed] [Google Scholar]
  9. Ford D. K. CULTURE OF HUMAN GENITAL "T-STRAIN" PLEUROPNEUMONIA-LIKE ORGANISMS. J Bacteriol. 1962 Nov;84(5):1028–1034. doi: 10.1128/jb.84.5.1028-1034.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gasser D. L. Current status of rat immunogenetics. Adv Immunol. 1977;25:93–139. [PubMed] [Google Scholar]
  11. Howard J. C., Scott D. W. The identification of sera distinguishing marrow-derived and thymus-derived lymphocytes in the rat thoracic duct. Immunology. 1974 Nov;27(5):903–922. [PMC free article] [PubMed] [Google Scholar]
  12. McLean I. W., Nakane P. K. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem. 1974 Dec;22(12):1077–1083. doi: 10.1177/22.12.1077. [DOI] [PubMed] [Google Scholar]
  13. Pickering W. J., Birch D. F. Bacteriologic and serologic findings in experimental pyelonephritis caused by Ureaplasma urealyticum. Infect Immun. 1989 Apr;57(4):1235–1239. doi: 10.1128/iai.57.4.1235-1239.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roberts J. A., Domingue G. J., Martin L. N., Kim J. C. Immunology of pyelonephritis in the primate model: live versus heat-killed bacteria. Kidney Int. 1981 Feb;19(2):297–305. doi: 10.1038/ki.1981.20. [DOI] [PubMed] [Google Scholar]
  15. Roberts J. A., Hardaway K., Kaack B., Fussell E. N., Baskin G. Prevention of pyelonephritis by immunization with P-fimbriae. J Urol. 1984 Mar;131(3):602–607. doi: 10.1016/s0022-5347(17)50513-3. [DOI] [PubMed] [Google Scholar]
  16. Silverblatt F. J. Host-parasite interaction in the rat renal pelvis: a possible role for pili in the pathogenesis of pyelonephritis. J Exp Med. 1974 Dec 1;140(6):1696–1711. doi: 10.1084/jem.140.6.1696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Torres V. E., Kramer S. A., Holley K. E., Hartman G. W., Bille J., Ruiz-Arguelles A., Ritts R. E. Effect of bacterial immunization on experimental reflux nephropathy. J Urol. 1984 Apr;131(4):772–776. doi: 10.1016/s0022-5347(17)50622-9. [DOI] [PubMed] [Google Scholar]

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