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Infection and Immunity logoLink to Infection and Immunity
. 1990 Nov;58(11):3802–3806. doi: 10.1128/iai.58.11.3802-3806.1990

Binding of viridans group streptococci to human platelets: a quantitative analysis.

P M Sullam 1, D G Payan 1, P F Dazin 1, F H Valone 1
PMCID: PMC313731  PMID: 2228249

Abstract

The binding of viridans group streptococci with human platelets was analyzed by two-color flow cytometry. Binding was detected within 15 s of mixing bacteria and platelets. At ratios of bacteria to platelets of 1:1, 10:1, 100:1, and 1,000:1, the percentages of bound streptococci (mean +/- standard deviation) were 93.2% +/- 5.4%, 80.0% +/- 8.6%, 39.8% +/- 11.1%, and 12.5% +/- 2.0%, respectively. Binding of labeled bacteria was reversed by adding a 500-fold excess of unlabeled streptococci. These results demonstrate that streptococcus-platelet binding is rapid, reversible, and saturable, which suggests a specific receptor-ligand interaction.

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Selected References

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