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. 1990 Dec;58(12):3899–3902. doi: 10.1128/iai.58.12.3899-3902.1990

Polymorphic expression of defensins in neutrophils from outbred rats.

P Eisenhauer 1, S S Harwig 1, D Szklarek 1, T Ganz 1, R I Lehrer 1
PMCID: PMC313752  PMID: 2254017

Abstract

We isolated and characterized a rat neutrophil defensin, RatNP-2, that differs from the previously described defensin RatNP-1 by containing Ser-7 in place of Arg-7. Although the resulting charge difference rendered RatNP-2 easily distinguishable from RatNP-1 on polyacrylamide gel electrophoresis gels, the two defensins exhibited very similar antimicrobial efficacies against Salmonella typhimurium, Staphylococcus aureus, and Candida albicans. The polymorphonuclear leukocytes of Sprague-Dawley rats obtained from one of two breeders also showed a marked polymorphism for defensin RatNP-4. This defensin was absent in two of seven animals and present in 1x or 2x relative amounts in the others. These observations indicate that a striking degree of defensin polymorphism exists in the polymorphonuclear leukocytes of outbred rodents.

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Selected References

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