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. 1990 Dec;58(12):4049–4054. doi: 10.1128/iai.58.12.4049-4054.1990

Establishment of a foreign antigen secretion system in mycobacteria.

K Matsuo 1, R Yamaguchi 1, A Yamazaki 1, H Tasaka 1, K Terasaka 1, M Totsuka 1, K Kobayashi 1, H Yukitake 1, T Yamada 1
PMCID: PMC313775  PMID: 1701418

Abstract

In order to develop recombinant Mycobacterium bovis BCG into a useful multivaccine vehicle, we established a foreign antigen secretion system in mycobacteria in which an extracellular alpha antigen of Mycobacterium kansasii was utilized as a carrier. By using this system, a B-cell epitope (Glu-12-Leu-Asp-Arg-Trp-Glu-Lys-Ile-19) of human immunodeficiency virus type 1 p17gag, which was identified by a fusion protein-based method, has been successfully obtained from BCG along with the alpha antigen. This is the first report of expression and secretion of a foreign viral antigen from BCG. It is possible that the system can become a universal vaccination vehicle applicable to protection against various infectious diseases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barré-Sinoussi F., Chermann J. C., Rey F., Nugeyre M. T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vézinet-Brun F., Rouzioux C. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):868–871. doi: 10.1126/science.6189183. [DOI] [PubMed] [Google Scholar]
  2. Bloom B. R. Learning from leprosy: a perspective on immunology and the Third World. J Immunol. 1986 Jul 1;137(1):i–x. [PubMed] [Google Scholar]
  3. Chain B. M., Kaye P. M., Shaw M. A. The biochemistry and cell biology of antigen processing. Immunol Rev. 1988 Dec;106:33–58. doi: 10.1111/j.1600-065x.1988.tb00772.x. [DOI] [PubMed] [Google Scholar]
  4. Davis A. R., Kostek B., Mason B. B., Hsiao C. L., Morin J., Dheer S. K., Hung P. P. Expression of hepatitis B surface antigen with a recombinant adenovirus. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7560–7564. doi: 10.1073/pnas.82.22.7560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans D. J., McKeating J., Meredith J. M., Burke K. L., Katrak K., John A., Ferguson M., Minor P. D., Weiss R. A., Almond J. W. An engineered poliovirus chimaera elicits broadly reactive HIV-1 neutralizing antibodies. Nature. 1989 Jun 1;339(6223):385-8, 340. doi: 10.1038/339385a0. [DOI] [PubMed] [Google Scholar]
  6. Husson R. N., James B. E., Young R. A. Gene replacement and expression of foreign DNA in mycobacteria. J Bacteriol. 1990 Feb;172(2):519–524. doi: 10.1128/jb.172.2.519-524.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jacobs W. R., Jr, Snapper S. B., Lugosi L., Bloom B. R. Development of BCG as a recombinant vaccine vehicle. Curr Top Microbiol Immunol. 1990;155:153–160. doi: 10.1007/978-3-642-74983-4_11. [DOI] [PubMed] [Google Scholar]
  8. Kagawa H., Gengyo K., McLachlan A. D., Brenner S., Karn J. Paramyosin gene (unc-15) of Caenorhabditis elegans. Molecular cloning, nucleotide sequence and models for thick filament structure. J Mol Biol. 1989 May 20;207(2):311–333. doi: 10.1016/0022-2836(89)90257-x. [DOI] [PubMed] [Google Scholar]
  9. Kieser T., Melton R. E. Plasmid pIJ699, a multi-copy positive-selection vector for Streptomyces. Gene. 1988 May 15;65(1):83–91. doi: 10.1016/0378-1119(88)90419-2. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Lotte A., Wasz-Höckert O., Poisson N., Dumitrescu N., Verron M., Couvet E. BCG complications. Estimates of the risks among vaccinated subjects and statistical analysis of their main characteristics. Adv Tuberc Res. 1984;21:107–193. [PubMed] [Google Scholar]
  12. Mackett M., Smith G. L., Moss B. Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415–7419. doi: 10.1073/pnas.79.23.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Matsuo K., Yamaguchi R., Yamazaki A., Tasaka H., Terasaka K., Yamada T. Cloning and expression of the gene for the cross-reactive alpha antigen of Mycobacterium kansasii. Infect Immun. 1990 Feb;58(2):550–556. doi: 10.1128/iai.58.2.550-556.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Matsuo K., Yamaguchi R., Yamazaki A., Tasaka H., Yamada T. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen. J Bacteriol. 1988 Sep;170(9):3847–3854. doi: 10.1128/jb.170.9.3847-3854.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Moore M. W., Carbone F. R., Bevan M. J. Introduction of soluble protein into the class I pathway of antigen processing and presentation. Cell. 1988 Sep 9;54(6):777–785. doi: 10.1016/s0092-8674(88)91043-4. [DOI] [PubMed] [Google Scholar]
  16. Newton S. M., Jacob C. O., Stocker B. A. Immune response to cholera toxin epitope inserted in Salmonella flagellin. Science. 1989 Apr 7;244(4900):70–72. doi: 10.1126/science.2468182. [DOI] [PubMed] [Google Scholar]
  17. Panicali D., Paoletti E. Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4927–4931. doi: 10.1073/pnas.79.16.4927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Papsidero L. D., Sheu M., Ruscetti F. W. Human immunodeficiency virus type 1-neutralizing monoclonal antibodies which react with p17 core protein: characterization and epitope mapping. J Virol. 1989 Jan;63(1):267–272. doi: 10.1128/jvi.63.1.267-272.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Popovic M., Sarngadharan M. G., Read E., Gallo R. C. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science. 1984 May 4;224(4648):497–500. doi: 10.1126/science.6200935. [DOI] [PubMed] [Google Scholar]
  20. Rauzier J., Moniz-Pereira J., Gicquel-Sanzey B. Complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum. Gene. 1988 Nov 30;71(2):315–321. doi: 10.1016/0378-1119(88)90048-0. [DOI] [PubMed] [Google Scholar]
  21. Romero P., Maryanski J. L., Corradin G., Nussenzweig R. S., Nussenzweig V., Zavala F. Cloned cytotoxic T cells recognize an epitope in the circumsporozoite protein and protect against malaria. Nature. 1989 Sep 28;341(6240):323–326. doi: 10.1038/341323a0. [DOI] [PubMed] [Google Scholar]
  22. Rüther U., Müller-Hill B. Easy identification of cDNA clones. EMBO J. 1983;2(10):1791–1794. doi: 10.1002/j.1460-2075.1983.tb01659.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shih M. F., Arsenakis M., Tiollais P., Roizman B. Expression of hepatitis B virus S gene by herpes simplex virus type 1 vectors carrying alpha- and beta-regulated gene chimeras. Proc Natl Acad Sci U S A. 1984 Sep;81(18):5867–5870. doi: 10.1073/pnas.81.18.5867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Snapper S. B., Lugosi L., Jekkel A., Melton R. E., Kieser T., Bloom B. R., Jacobs W. R., Jr Lysogeny and transformation in mycobacteria: stable expression of foreign genes. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6987–6991. doi: 10.1073/pnas.85.18.6987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Suzuki Y., Yoshinaga K., Ono Y., Nagata A., Yamada T. Organization of rRNA genes in Mycobacterium bovis BCG. J Bacteriol. 1987 Feb;169(2):839–843. doi: 10.1128/jb.169.2.839-843.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tasaka H., Matsuo Y. Specificity and distribution of alpha antigens of Mycobacterium kansasii and Mycobacterium marinum. Am Rev Respir Dis. 1984 Oct;130(4):647–649. doi: 10.1164/arrd.1984.130.4.647. [DOI] [PubMed] [Google Scholar]
  27. Terasaka K., Yamaguchi R., Matsuo K., Yamazaki A., Nagai S., Yamada T. Complete nucleotide sequence of immunogenic protein MPB70 from Mycobacterium bovis BCG. FEMS Microbiol Lett. 1989 Apr;49(2-3):273–276. doi: 10.1016/0378-1097(89)90052-9. [DOI] [PubMed] [Google Scholar]
  28. Thole J. E., van Schooten W. C., Keulen W. J., Hermans P. W., Janson A. A., de Vries R. R., Kolk A. H., van Embden J. D. Use of recombinant antigens expressed in Escherichia coli K-12 to map B-cell and T-cell epitopes on the immunodominant 65-kilodalton protein of Mycobacterium bovis BCG. Infect Immun. 1988 Jun;56(6):1633–1640. doi: 10.1128/iai.56.6.1633-1640.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yamaguchi R., Matsuo K., Yamazaki A., Abe C., Nagai S., Terasaka K., Yamada T. Cloning and characterization of the gene for immunogenic protein MPB64 of Mycobacterium bovis BCG. Infect Immun. 1989 Jan;57(1):283–288. doi: 10.1128/iai.57.1.283-288.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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