FIGURE 6.

None of the mutations produced drastic effects on the overall structure of TbADAT2/3 and its variants. Both wild-type recombinant TbADAT2/3 and the various mutants from Tables 1 and 2 were analyzed by circular dichroism. The UV spectra were taken at various wavelengths between 200 and 260 nm. Molar elipticity ([θ]) was calculated as described in Materials and Methods and plotted as a function of wavelength. None of the mutants had significant spectral deviations when compared to the wild type, indicating that no major structural rearrangements had taken place.