FIGURE 7.
TbADAT2 C-terminal mutant still heterodimerizes with ADAT3. (A) Wild-type TbADAT2/3 and the two C-terminal mutants were fractionated by size-exclusion chromatography as indicated in the Materials and Methods section. Both mutants eluted with a size consistent with that calculated for the wild type (∼68 kDa), again suggesting that these mutations cause no major effects on the multimeric state of these mutants. (B) Kav vs. molecular weight standard curve. The standards and their respective molecular weights are as marked (gray diamonds) and labeled in gray. The wild-type and mutant ADAT2/3 enzymes are marked (black circles) and labeled in black.