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. 1989 Jul;57(7):2256–2259. doi: 10.1128/iai.57.7.2256-2259.1989

Binding of Escherichia coli S fimbriae to cultured human endothelial cells.

J Parkkinen 1, A Ristimäki 1, B Westerlund 1
PMCID: PMC313870  PMID: 2567284

Abstract

The binding of Escherichia coli adhesins to human umbilical vein endothelial cells was studied by a cell monolayer enzyme-linked immunosorbent assay. S fimbriae displayed a concentration-dependent and saturable binding to the endothelial cells which was mediated by their sialylgalactoside-specific lectin activity. P fimbriae exhibited only low binding, and type 1 fimbriae exhibited no binding to these cells.

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Selected References

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  1. Edén C. S., Hanson L. A., Jodal U., Lindberg U., Akerlund A. S. Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection. Lancet. 1976 Sep 4;1(7984):490–492. [PubMed] [Google Scholar]
  2. Firon N., Ofek I., Sharon N. Interaction of mannose-containing oligosaccharides with the fimbrial lectin of Escherichia coli. Biochem Biophys Res Commun. 1982 Apr 29;105(4):1426–1432. doi: 10.1016/0006-291x(82)90947-0. [DOI] [PubMed] [Google Scholar]
  3. Gaastra W., de Graaf F. K. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiol Rev. 1982 Jun;46(2):129–161. doi: 10.1128/mr.46.2.129-161.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hacker J., Schmidt G., Hughes C., Knapp S., Marget M., Goebel W. Cloning and characterization of genes involved in production of mannose-resistant, neuraminidase-susceptible (X) fimbriae from a uropathogenic O6:K15:H31 Escherichia coli strain. Infect Immun. 1985 Feb;47(2):434–440. doi: 10.1128/iai.47.2.434-440.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hagberg L., Hull R., Hull S., Falkow S., Freter R., Svanborg Edén C. Contribution of adhesion to bacterial persistence in the mouse urinary tract. Infect Immun. 1983 Apr;40(1):265–272. doi: 10.1128/iai.40.1.265-272.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jaffe E. A., Hoyer L. W., Nachman R. L. Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest. 1973 Nov;52(11):2757–2764. doi: 10.1172/JCI107471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karlsson K. A. Animal glycolipids as attachment sites for microbes. Chem Phys Lipids. 1986 Dec 15;42(1-3):153–172. doi: 10.1016/0009-3084(86)90050-2. [DOI] [PubMed] [Google Scholar]
  8. Korhonen T. K., Nurmiaho E. L., Ranta H., Edén C. S. New Method for isolation of immunologically pure pili from Escherichia coli. Infect Immun. 1980 Feb;27(2):569–575. doi: 10.1128/iai.27.2.569-575.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Korhonen T. K., Parkkinen J., Hacker J., Finne J., Pere A., Rhen M., Holthöfer H. Binding of Escherichia coli S fimbriae to human kidney epithelium. Infect Immun. 1986 Nov;54(2):322–327. doi: 10.1128/iai.54.2.322-327.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Korhonen T. K., Valtonen M. V., Parkkinen J., Väisänen-Rhen V., Finne J., Orskov F., Orskov I., Svenson S. B., Mäkelä P. H. Serotypes, hemolysin production, and receptor recognition of Escherichia coli strains associated with neonatal sepsis and meningitis. Infect Immun. 1985 May;48(2):486–491. doi: 10.1128/iai.48.2.486-491.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Korhonen T. K., Virkola R., Holthöfer H. Localization of binding sites for purified Escherichia coli P fimbriae in the human kidney. Infect Immun. 1986 Nov;54(2):328–332. doi: 10.1128/iai.54.2.328-332.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. O'Hanley P., Lark D., Falkow S., Schoolnik G. Molecular basis of Escherichia coli colonization of the upper urinary tract in BALB/c mice. Gal-Gal pili immunization prevents Escherichia coli pyelonephritis in the BALB/c mouse model of human pyelonephritis. J Clin Invest. 1985 Feb;75(2):347–360. doi: 10.1172/JCI111707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. O'Hanley P., Low D., Romero I., Lark D., Vosti K., Falkow S., Schoolnik G. Gal-Gal binding and hemolysin phenotypes and genotypes associated with uropathogenic Escherichia coli. N Engl J Med. 1985 Aug 15;313(7):414–420. doi: 10.1056/NEJM198508153130704. [DOI] [PubMed] [Google Scholar]
  14. Ogawa S. K., Yurberg E. R., Hatcher V. B., Levitt M. A., Lowy F. D. Bacterial adherence to human endothelial cells in vitro. Infect Immun. 1985 Oct;50(1):218–224. doi: 10.1128/iai.50.1.218-224.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Parkkinen J., Finne J. Isolation of sialyl oligosaccharides and sialyl oligosaccharide phosphates from bovine colostrum and human urine. Methods Enzymol. 1987;138:289–300. doi: 10.1016/0076-6879(87)38024-3. [DOI] [PubMed] [Google Scholar]
  16. Parkkinen J., Korhonen T. K., Pere A., Hacker J., Soinila S. Binding sites in the rat brain for Escherichia coli S fimbriae associated with neonatal meningitis. J Clin Invest. 1988 Mar;81(3):860–865. doi: 10.1172/JCI113395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Parkkinen J., Rogers G. N., Korhonen T., Dahr W., Finne J. Identification of the O-linked sialyloligosaccharides of glycophorin A as the erythrocyte receptors for S-fimbriated Escherichia coli. Infect Immun. 1986 Oct;54(1):37–42. doi: 10.1128/iai.54.1.37-42.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Riegman N., van Die I., Leunissen J., Hoekstra W., Bergmans H. Biogenesis of F7(1) and F7(2) fimbriae of uropathogenic Escherichia coli: influence of the FsoF and FstFG proteins and localization of the Fso/FstE protein. Mol Microbiol. 1988 Jan;2(1):73–80. [PubMed] [Google Scholar]
  19. Rodriguez-Ortega M., Ofek I., Sharon N. Membrane glycoproteins of human polymorphonuclear leukocytes that act as receptors for mannose-specific Escherichia coli. Infect Immun. 1987 Apr;55(4):968–973. doi: 10.1128/iai.55.4.968-973.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Saukkonen K. M., Nowicki B., Leinonen M. Role of type 1 and S fimbriae in the pathogenesis of Escherichia coli O18:K1 bacteremia and meningitis in the infant rat. Infect Immun. 1988 Apr;56(4):892–897. doi: 10.1128/iai.56.4.892-897.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Väisänen V., Elo J., Tallgren L. G., Siitonen A., Mäkelä P. H., Svanborg-Edén C., Källenius G., Svenson S. B., Hultberg H., Korhonen T. Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet. 1981 Dec 19;2(8260-61):1366–1369. doi: 10.1016/s0140-6736(81)92796-3. [DOI] [PubMed] [Google Scholar]

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