Fig. 1.

Scheme for pMAK17 construction. a Preparation of pCMV. b Preparation of PA. c, d Preparation of rtTA, PURO, and DsRED2, which were then sub-cloned into pBS. e An inverse PCR to generate pTRE-Tight. f Preparation of EGFP. g Preparation of pTRE-EGFP. h Preparation of PA to change a restriction enzyme site from XhoI to KpnI. i Sub-cloning EGFP with P_Tight and PA to pBS-DsRED2. j An inverse PCR for pMAK15. k Preparation of pMAK15. l Preparation of PURO with P_CMV and PA for pMAK16. m Preparation of pMAK16. n Preparation of PURO with rtTA and PA for pMAK17. o Preparation of pMAK17. p Preparation of MCSs for pMAK42. q Preparation of pMAK42