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. 2011 Jul 21;5:42. doi: 10.3389/fnana.2011.00042

Spinogenesis and Pruning in the Anterior Ventral Inferotemporal Cortex of the Macaque Monkey: An Intracellular Injection Study of Layer III Pyramidal Cells

Guy N Elston 1,*, Tomofumi Oga 2, Tsuguhisa Okamoto 2, Ichiro Fujita 2
PMCID: PMC3143722  PMID: 21811440

Abstract

Pyramidal cells grow and mature at different rates among different cortical areas in the macaque monkey. In particular, differences across the areas have been reported in both the timing and magnitude of growth, branching, spinogenesis, and pruning in the basal dendritic trees of cells in layer III. Presently available data suggest that these different growth profiles reflect the type of functions performed by these cells in the adult brain. However, to date, studies have focused on only a relatively few cortical areas. In the present investigation we quantified the growth of the dendritic trees of layer III pyramidal cells in the anterior ventral portion of cytoarchitectonic area TE (TEav) to better comprehend developmental trends in the cerebral cortex. We quantified the growth and branching of the dendrities, and spinogenesis and pruning of spines, from post-natal day 2 (PND2) to four and a half years of age. We found that the dendritic trees increase in size from PND2 to 7 months of age and thereafter became smaller. The dendritic trees became increasingly more branched from PND2 into adulthood. There was a two-fold increase in the number of spines in the basal dendritic trees of pyramidal cells from PND2 to 3.5 months of age and then a 10% net decrease in spine number into adulthood. Thus, the growth profile of layer III pyramidal cells in the anterior ventral portion of the inferotemporal cortex differs to that in other cortical areas associated with visual processing.

Keywords: plasticity, Hebb, dendrite, spine, maturation, cortex, development

Introduction

While synaptogenesis peaks in the first few months after birth in the macaque monkey cerebral cortex (Rakic et al., 1986; Bourgeois et al., 1994), pyramidal cells in some cortical areas appear to continue to grow longer dendrites, increasing their dendritic territories, become more branched, and continue to grow spines over a period of years. Layer III pyramidal cells in inferotemporal cortex (IT), for example, continue to grow larger dendritic trees well beyond the peak in synaptogenesis into adulthood (Elston et al., 2010a) as do those in prefrontal cortex (Cupp and Uemura, 1980; Elston et al., 2009). Protracted synaptogenesis and dendritic growth has also been reported in prefrontal cortex in human (Huttenlocher, 1990; Huttenlocher and Dabholkar, 1997; Conel, 1967). However, those in the primary visual area (V1) of the macaque monkey become progressively smaller from birth into adulthood, with as much as a 30% decrease in size of their dendritic trees during this time (Boothe et al., 1979; Elston et al., 2010a). Those in the primary auditory cortex (A1) of the macaque grow from birth until 7 months of age, thereafter they decrease in size into adulthood (Elston et al., 2010b).

The diversity in growth profiles of this single neuronal type, the pyramidal cell, in different cortical areas suggest that genetic and epigenetic mechanisms that induce and modulate neurite growth vary across the cortical mantle, as do those that control dendrite and spine atrophy. Recently there has been considerable focus on regional expression of molecular markers across the cortical mantle, and how they may influence arealization in cortex (Oldham et al., 2006; Kudo et al., 2007; Mühlfriedel et al., 2007; Rakic, 2010). As the list of molecular markers expands into the future it will be beneficial to be able to compare expression of such markers with aspects of neuronal morphology and intrinsic connectivity among cortical areas. Here we expand the basis for comparison by studying the growth of basal dendritic trees of layer III pyramidal cells in anterior ventral IT of the macaque monkey (area TEav). TEav is a major projection target of more dorsal and posterior TE, and lies at the interface between the ventral visual pathway and the medial temporal lobe areas (perirhinal, entorhinal, and hippocampal areas; Miyashita, 1993; Suzuki and Amaral, 2003). TEav differs from more dorsal or posterior TE in terms of functions (visual memory vs. visual perceptual function), and connections (Ungerleider and Desimone, 1986; Yukie, 1997; Saleem and Hashikawa, 1998) and is crucial for visual recognition (Bachevalier et al., 1993; Mishkin and Murray, 1994; Burwell et al., 1995; Murray and Bussey, 1999; Suzuki and Amaral, 2003). We found that relative changes in the size of the basal dendritic trees of layer III pyramidal cells in TEav from post-natal day 2 (PND2) into adulthood differed from those in other cortical areas involved in visual processing, as did changes in branching structure. There was a two-fold increase in the number of spines in the basal dendritic trees of pyramidal cells from PND2 to 3.5 months of age and then a 10% net decrease in spine number into adulthood, which differed to that in TEpd (threefold increase and a 40% decrease) and V1 (twofold increase and a >75% decrease) over the same period.

Materials and Methods

Four male macaque monkeys (Macaca fascicularis) aged from 2 days old to 4.5 years of age were used in the present study (Table 1). Data were sampled from visual, auditory, association, and prefrontal cortex of these same animals and have been reported elsewhere (Elston et al., 2009, 2010a,b). We have outlined in detail in previous studies the cell injection methodology and immunohistochemical processing employed in the present study (Buhl and Schlote, 1987; Elston and Rosa, 1997; Elston, 2001). Briefly, animals were overdosed with sodium pentobarbital following unrelated extracellular recording experiments, perfused intracardially and the brain removed. Blocks of tissue were excised from the anterior inferior temporal pole, trimmed, “flat-mounted,” and postfixed overnight between glass slides in a solution of 4% paraformaldehyde in 0.1 M phosphate buffer (PB; pH 7.2; Figure 1). Serial 250 μm sections were cut with the aid of a vibratome tangential to the cortical surface. Sections were then immersed in 10−5 mol/L 4,6 diamidino-2-phenylindole (DAPI; Sigma D9542, St Louis, MO, USA) in PB at room temperature for approximately 10 min prior to being mounted between Millipore filters (AABG02500, Billerica, MA, USA).

Table 1.

Age, weight, gender, and number of pyramidal cells injected in the animals used in the present study.

Age Animal Gender Body weight (kg) No. of cells
2 Days CI 9 Male 0.35 23
3.5 Months CI 10 Male 0.56 29
7 Months CI 8 Male 0.7 28
4.5 Years CI 12 Male 2.7 26
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Figure 1.

Figure 1

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(A) Schematic illustrating cortical maps published by Saleem et al. (2000) and Suzuki and Amaral (1994). Note, in particular, differences in the location of the borders of areas TE and 36. Note also that area TE has been subdivided by Saleem and colleagues to include a posterior and anterior region (TEp and TEa, respectively), and that the anterior region has been divided into dorsal and ventral regions (TEad and TEav, respectively). (B) Slice preparations for intracellular injection were prepared by opening up the anterior middle temporal sulcus (amts), posterior middle temporal sulcus (pmts), and the occipito-temporal sulcus (ots) and flattening the tissue between glass. The tissue was left overnight in paraformaldehyde and sectioned the following day. (C) Cells were injected in cortex medial to the amts and lateral to the anterior tip of the ots. Cells in the present investigation were sampled in area TEav of Saleem et al. (2000), including area 36c of Suzuki and Amaral (1994). sts: superior temporal sulcus, A,D,L: anterior, dorsal, and lateral, respectively.

DAPI-labeled neurons were visualized by UV excitation (380–420 nm) on a fixed-stage Nikon Eclipse FN1 microscope (Tokyo, Japan) and injected with Lucifer Yellow with continuous current (up to 100 nA) under visual guidance. We injected cells with cell bodies located at the base of layer III so as to be able to make direct comparisons with our other data sampled from visual, auditory, and prefrontal cortex (Elston et al., 2009, 2010a,b). We use the nomenclature of Hassler for cortical layers (Hassler, 1966). See Casagrande and Kaas (1994), Elston and Rosa, 1998) for a critique. All cells were injected in cortex medial to the anterior middle temporal sulcus and lateral to the anterior tip of the occipito-temporal sulcus (Figure 1). The location of the borders between cortical areas in this region remains controversial (e.g., Suzuki and Amaral, 1994; Saleem et al., 2000). Cells in the present investigation were sampled in area TEav of Saleem et al. (2000), including area 36c of Suzuki and Amaral (1994; Figure 1). Once approximately 50 neurons had been injected, the slice was processed for a light-stable reaction product (Elston and Rosa, 1997). Specifically, the sections were processed in 1:400,000 biotinylated anti-Lucifer Yellow (A-5751, Invitrogen) in stock solution [2% bovine serum albumin (Sigma A3425), 1% Triton X-100 (Sigma X100), and 5% sucrose in PB] for 5 days at room temperature, washed three times in PB, then incubated in 3,3′-diamino-benzidine (DAB; Sigma D 5637; 1:200 in PB) for 5 min at room temperature before being reacted in 1% hydrogen peroxide and 0.5% DAB in PB (Figure 2).

Figure 2.

Figure 2

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(A) Low and (B) intermediate power photomicrographs of layer III pyramidal cells sampled from the anterior ventral portion of cytoarchitectonic area TE that were injected with Lucifer Yellow and processed with a diamino-benzidine reaction product. (C–E) High power photomicrographs of the basal dendrites of layer III pyramidal cells sampled from TEav illustrating differences in spine density at different ages. Scale bar = 50 μm in (A), 25 μm in (B), and 10 μm in (C–E).

Neurons were reconstructed in three dimensions with the aid of Neurolucida system (MBF Bioscience, Williston, VT, USA) coupled with a Nikon Eclipse 80i microscope equipped with a motorized stage (Ludl Electronic Products, Hawthorne, NY, USA) and a CCD camera (CX9000, MBF Biosciences). The size and branching structure of the basal dendritic trees was determined with the aid of Matlab software (Mathworks, Natick, MA, USA). Spine densities were calculated by drawing the entire dendrite of randomly selected cells with the aid of a Nikon ×100 oil immersion objective (numerical aperture = 1.40) coupled with a camera lucida, and labeling each spine along the dendrite. The number of spines was counted per 10 μm of dendrite in the drawings (Eayrs and Goodhead, 1959; Valverde, 1967). Horizontally projecting dendrites were selected to avoid trigonometric error. All spines were drawn and no distinction was made between different types of spines. Statistical comparisons were made using Matlab software. Methodologies applied here accord with protocols approved by Osaka University and regulations for the care and use of animals set out by the NIH (publication No. 86-23, revised 1996).

Results

Two hundred and thirty pyramidal cells were injected in layer III of cortex for the present investigation. One hundred and six of these cells were included for analyses as they satisfied inclusion criteria (Table 1): namely, they (1) had an unambiguous apical dendrite, (2) had their complete basal dendritic trees contained within the section, and (3) were well filled. From these cells we drew and tallied over 31,000 individual dendritic spines. These data are presented and compared with those obtained previously from V1 and the posterior dorsal region of area TE (TEpd; Elston et al., 2009, 2010a) to allow comparisons of the developmental profiles of cells in these different cortical areas.

Dendritic tree size

Pyramidal cells at PND2 (6.80 × 104 μm ± 1.56 × 104 μm, mean ± SD) continued to grow through 3.5 months of age (7.76 × 104 μm ± 1.46 × 104 μm) and 7 months of age (11.54 × 104 μm ± 2.11 × 104 μm; Figure 3A). Cells in the adult (8.54 × 104 μm ± 1.51 × 104 μm) were 26% smaller than those observed at 7 months of age, suggesting a peak size had been obtained and some pruning had occurred. This profile differed to that observed in TEpd, where the dendritic trees of pyramidal cells continued to grow from PND through to adulthood. Moreover, this profile differed to that observed in V1, where cells became progressively smaller from PND2 to 3.5 months, 7 months, and adulthood. Statistical analysis (one-way ANOVAs) revealed that the size of the basal dendritic trees of pyramidal cells in TEav were significantly different (p < 0.05) among the age groups [F(3) = 39.46].

Figure 3.

Figure 3

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Plots of size (A), number of branching points (B), peak spine density (C), and total number of spines (D) in the basal dendritic trees of layer III pyramidal neurons in TEav sampled from animals 2 days old (2D), three and a half months old (3.5 month), 7 months old (7 month), and 4.5 years old (Ad). Error bars = SD.

Branching patterns

Sholl analyses revealed differences in the branching complexity of neurons in TEav with aging (Figure 3B). There was a trend for increasing complexity in the branching of the basal dendritic trees of neurons, as revealed by the average peak complexity from newborn (31.96 ± 6.92) through 3.5 months of age (30.24 ± 5.92), 7 months of age (33.57 ± 9.04) to adulthood (34.19 ± 5.35). Statistical analysis (repeated measures ANOVA) revealed no significant difference (p > 0.05) in the number of branches in the basal dendritic trees of pyramidal cells across the age groups (F(3) = 1.79).

Spine densities of the basal dendrites

The peak spine density per 10 μm dendritic segment at PND2 (20.1 ± 4.4, mean ± SD) was less than that at 3.5 months of age (39.3 ± 7.6; Figure 3C). The peak spine density at 7 months of age was (33.5 ± 4.9), more than that observed in the adult (32.0 ± 6.9). A repeated measures ANOVA revealed these differences in spine density to be significantly different across ages [p < 0.05, F(3) = 79.63]. This trend was similar to that observed in both V1 and TEpd in so much as the peak was observed at 3.5 months of age; however, the magnitude of change differs among cortical areas.

By summing the product of the number of dendritic branches and spine density along the entire extent of the dendritic tree we were able to calculate an estimate of the total number of spines in the basal dendritic tree of the “average” pyramidal neuron in TEav for the different age groups (Elston, 2001). These calculations revealed a considerable increase in the number of spines in the basal dendritic trees of cells from PND2 (5,118) to 3.5 months of age (12,289). At 7 months of age, cells had 12,084 spines in their basal dendritic trees, more than observed in the adult (10,910; Figure 3D).

Discussion

Here we report on the morphology of the basal dendritic trees of layer III pyramidal cells in the anterior ventral IT in macaque monkeys aged 2 days to 4.5 years old. The dendritic trees of these cells became progressively larger from birth to 7 months of age, thereafter diminishing in size to adulthood. The dendritic trees of these cells became progressively more branched from PND2 to adulthood. Spine density increased from PND2 to 3.5 months of age, thereafter decreasing into adulthood. Our calculations reveal that these cells, on average, have 5,328 spines within their basal dendritic trees at PND2, increasing to 12,289 at 3.5 months of age before decreasing to 10,910 spines in early adulthood. Thus, considerably more spines are grown within the dendritic trees of these cells than lost over this period.

Comparison of the growth profiles of the dendritic trees of pyramidal cells in TEav with those in layer III in other cortical areas reveal some interesting similarities and differences. It is becoming increasingly clear that not all neocortical pyramidal cells grow at the same rate, nor to the same extent. In addition, it is becoming increasing apparent that different aspects of dendritic growth may occur independently, such as the tangential spread of the dendritic trees, the number of dendritic branches sprouted, and the spine density along the dendrites. Of particular note here is that the dendritic trees of layer III pyramidal cells in TEav continue to become more branched from 7 months of age to adulthood during which time their dendritic trees diminish in size (Figures 3A,B). This observation differs to those in all other cortical areas studied to date, including visual (V1, V2, V4, TEO, TEpd), auditory (A1), and dorsolateral granular prefrontal cortex (area 12vl; Elston et al., 2009, 2010a,b). Moreover, the presence of increasing numbers of dendritic branches from 3.5 months of age to 7 months of age into adulthood, which are studded with dendritic spines, suggest that spinogenesis continues to occur on dendrites in the developing brain well beyond the peak period of spinogenesis reported at 3.5 months of age (Elston et al., 2009, 2010a,b). The magnitude and profile of spine acquisition and loss in the dendritic trees of pyramidal cells also varies among cortical areas (Figure 4A). In TEav there is a two-fold increase in the number of spines in the basal dendritic trees of pyramidal cells from PND2 to 3.5 months of age and then a 10% net decrease in spine number into adulthood (Figure 4B). In TEpd there is a three-fold increase and a 40% decrease in the number of spines in the basal dendritic trees of pyramidal cells over the same period (Elston et al., 2010a). In V1 there is a two-fold increase and a >75% decrease in the number of spines in the basal dendritic trees of pyramidal cells over this period (Elston et al., 2010a).

Figure 4.

Figure 4

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(A) Plot illustrating how different aspects of basal dendritic tree structure, such as the size and branching structure, of layer III pyramidal neurons in TEav may vary independently with aging. For example, from 2 days of age (2D) to three and a half months of age (3.5 month) there is a decrease in branching complexity coupled with an increase in the size of the dendritic trees. From 7 months of age (7 month) to four and a half years of age (AD) there is an increase in branching complexity coupled with a decrease in the size of the dendritic trees. (B) Plots of net change in number of spines in the basal dendritic trees of pyramidal cells in TEav, TEpd, and V1 from 2 days of age into adulthood. Note there is a net gain in the number of spines in the basal dendritic trees of cells in high level association areas TEav and TEpd, but a net loss of spines in area V1. A net loss in the total number of spines in the basal dendritic trees of layer III pyramidal cells has also been reported in the primary auditory area over the same period (Elston et al., 2010b). Error bars = SD.

Perhaps it is not surprising to find that growth profiles of pyramidal neurons differ within TE, or between cells in TE and those in other cortical areas, as these cortical areas are characterized by different patterns of connectivity, neuronal composition, chemoarchitectonic profiles, and function. For example, these regions are characterized by different patterns of connectivity via the callosum (Van Essen et al., 1982) and with the amygdala (Iwai et al., 1987), differences have been reported in the density of neurons immunoreactive for SMI-32 (Hof et al., 1995) and calbindin (Kondo et al., 1999), and patterns of inhibitory connections and pyramidal cell connections vary among cortical areas (Amir et al., 1993; Lund et al., 1993; Fujita and Fujita, 1996; DeFelipe et al., 1999; Tanigawa et al., 2005). Phosphorylation levels of protein kinase C (F1/50 kDa) and opiate and glutamate receptor subtype densities also vary among these cortical areas (Lewis et al., 1981; Nelson et al., 1987).

In future studies it will be worthwhile to study the developmental profiles of the apical dendrites of these pyramidal cells among cortical areas, pyramidal cells in other cortical layers, and inhibitory interneurons. Only then will we have a better appreciation of the extent and significance of specializations in the patterns of connectivity and complexity in circuitry among cortical areas in the normal developing and mature neocortex, and how abnormalities in microstructure may result in functional disorders (see Elston, 2002, 2007; Jacobs and Scheibel, 2002; Levitt, 2005; Treves, 2005; Spruston, 2008; Rakic, 2010 for reviews).

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

Supported by grants from the Japan Science and Technology Agency (Core Research for Evolutional Science and Technology), Osaka University, the Ministry of Education, Culture, Sports, Science, and Technology (17022025, 23240047), and the I Hear innovation Foundation (Australia). Thanks go to Andrea Machado for assistance with quantifying spine densities. Address correspondence and reprint requests to Guy Elston at Centre for Cognitive Neuroscience, 60 Duke Road, Doonan, QLD 4562, Australia.

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