Table 5.

Rate constants for select binding pocket, tertiary contact, and P1 mutants.

RNA	Kd (nM)	kon (M−1 min−1)	koff (min−1)
Wild-type	0.011	1.0 ± 0.016 × 106	1.1 ± 0.082 × 10−5
C92Ua	15 ± 1.1	8.0 ± 0.99 × 105	1.0 ± 0.16 × 10−2
G20A	0.21 ± 0.067	9.1 ± 1.8 × 105	2.2 ± 0.077 × 10−4
C44U/G83A	190 ± 8.6	8.7 ± 1.2 × 104	2.5 ± 0.25 × 10−2
Vc2 Δ95	63 ± 13	2 × 105 b	1.3 ± 0.10 × 10−2
Vc2 Δ96	5.4 ± 0.59	2 × 106 b	1.0 ± 0.10 × 10−2
Vc2 Δ98	5.9 ± 0.79	5.0 ± 1.0 × 105	3 × 10−3 b
Vc2 Δ99	< 0.8c	1.3 ± 0.40 × 106	-
Vc2 Δ100	< 0.5c	1.2 ± 0.30 × 106	-

^aAll values reported for C92U were reported previously (21).

^bCalculated from the equation K_d=k_off/k_on.

^cThese values could not be measured using equilibrium methods and upper estimates are given.