Abstract
Wachsman, J. T. (University of Illinois, Urbana) and R. Mangalo. Use of 8-azaguanine for the isolation of auxotrophic mutants of Bacillus megaterium. J. Bacteriol. 83:35–37. 1962.—8-Azaguanine is bactericidal for Bacillus megaterium strain KM, primarily under conditions where growth is possible. This analogue has been used to enrich for auxotrophic mutants in ultraviolet-irradiated populations. The survivors were found to contain from 1.6 to 33.0% amino acid auxotrophs and from 19 to 29% pyrimidine nucleoside auxotrophs. Since many different biological systems are sensitive to 8-azaguanine, the use of this analogue to enrich for auxotrophic mutants may have a more general application than techniques currently employed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAUMAN N., DAVIS B. D. Selection of auxotrophic bacterial mutants through diaminopimelic acid or thymine deprival. Science. 1957 Jul 26;126(3265):170–170. doi: 10.1126/science.126.3265.170. [DOI] [PubMed] [Google Scholar]
- GORINI L., KAUFMAN H. Selecting bacterial mutants by the penicillin method. Science. 1960 Feb 26;131(3400):604–605. doi: 10.1126/science.131.3400.604. [DOI] [PubMed] [Google Scholar]
- Iyer V. CONCENTRATION AND ISOLATION OF AUXOTROPHIC MUTANTS OF SPOREFORMING BACTERIA. J Bacteriol. 1960 Feb;79(2):309–310. doi: 10.1128/jb.79.2.309-310.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUBIN M. Selection of auxotrophic bacterial mutants by tritium-labeled thymidine. Science. 1959 Mar 27;129(3352):838–839. doi: 10.1126/science.129.3352.838. [DOI] [PubMed] [Google Scholar]
- MANGALO R., WACHSMAN J. T. Effect of 8-azaguanine on growth and viability of Bacillus megaterium. J Bacteriol. 1962 Jan;83:27–34. doi: 10.1128/jb.83.1.27-34.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMITH J. D., MATTHEWS R. E. The metabolism of 8-azapurines. Biochem J. 1957 Jun;66(2):323–333. doi: 10.1042/bj0660323. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zamenhof S. EFFECTS OF HEATING DRY BACTERIA AND SPORES ON THEIR PHENOTYPE AND GENOTYPE. Proc Natl Acad Sci U S A. 1960 Jan;46(1):101–105. doi: 10.1073/pnas.46.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]