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Expression profiles of thyrotropin receptor (TSHR) variants. HEK-EM293 cells were transfected with plasmids encoding the listed TSHR variants. After 48 hours, all were probed with 2C11 or 4C1 antibody, both specific to two different epitopes within the extracellular portion of TSHR. All variants showed reduced cell surface presentation compared with wild-type TSHR. Bars represent mean ± standard error of the mean (SEM).
