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. 1964 Oct;88(4):817–820. doi: 10.1128/jb.88.4.817-820.1964

METABOLIC NONEQUIVALENCE OF THE TWO GLUCOSE MOIETIES OF CELLOBIOSE IN CELLVIBRIO GILVUS

Elizabeth J Swisher a, Waldemar O Storvick a,1, Kendall W King a
PMCID: PMC314819  PMID: 14219041

Abstract

Swisher, Elizabeth J. (Virginia Polytechnic Institute, Blacksburg), Waldemar O. Storvick, and Kendall W. King. Metabolic nonequivalence of the two glucose moieties of cellobiose in Cellvibrio gilvus. J. Bacteriol. 88:817–820. 1964.—Cellobiose was synthesized in 40% yield with uniform C14 labeling in the reducing glucose moiety and no label in the nonreducing glucosyl. Resting-cell suspensions of Cellvibrio gilvus respiring the labeled cellobiose derived approximately 80% of their respiratory CO2 from the reducing glucosyl and 20% from the nonreducing glucose. Analysis of isotope content in CO2 from cells respiring a mixture of labeled cellobiose and unlabeled glucose confirmed that the glucose-1-phosphate produced from phosphorolysis of cellobiose is less extensively converted to CO2 than is either the glucose released by phosphorolysis of cellobiose or glucose absorbed from the medium. In crude cell extracts, release of glucose from cellobiose was shown to be Pi-dependent, the pH optimum of cellobiose phosphorylase being 6.2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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