Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1964 Oct;88(4):1084–1086. doi: 10.1128/jb.88.4.1084-1086.1964

PRODUCTION OF PROTOPLASTS IN AN OSMOTIC MUTANT OF NEUROSPORA CRASSA WITHOUT ADDED ENZYME

James G Hamilton 1, Janet Calvet 2
PMCID: PMC314858  PMID: 14219023

Abstract

Hamilton, James G. (Tulane University School of Medicine, New Orleans, La.), and Janet Calvet. Production of protoplasts in an osmotic mutant of Neurospora crassa without added enzyme. J. Bacteriol. 88:1084–1086. 1964.—An osmotic mutant of Neurospora crassa was grown in minimal medium containing increasing concentrations of sorbose. Protoplasts were produced in the higher concentrations by inhibition of cell-wall synthesis. Similar molar concentrations of fructose, glucose, and sucrose also produced protoplasts, suggesting a relationship between inhibition of cell-wall synthesis and osmotic pressure. Increased sugar concentration led to decreased growth.

Full text

PDF
1084

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BACHMANN B. J., BONNER D. M. Protoplasts from Neurospora crassa. J Bacteriol. 1959 Oct;78:550–556. doi: 10.1128/jb.78.4.550-556.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DE TERRA N., TATUM E. L. Colonial growth of Neurospora. Sorbose and enzymes alter the composition of the cell wall and induce morphological changes. Science. 1961 Oct 13;134(3485):1066–1068. doi: 10.1126/science.134.3485.1066. [DOI] [PubMed] [Google Scholar]
  3. Emerson S., Emerson M. R. PRODUCTION, REPRODUCTION, AND REVERSION OF PROTOPLAST-LIKE STRUCTURES IN THE OSMOTIC STRAIN OF NEUROSPORA CRASSA. Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):668–671. doi: 10.1073/pnas.44.7.668. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Perkins D D. New Markers and Multiple Point Linkage Data in Neurospora. Genetics. 1959 Nov;44(6):1185–1208. doi: 10.1093/genetics/44.6.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES