Abstract
Hoffman, Heiner (New York University, New York, N.Y.), and Michael E. Frank. ”Germination tube” growth in Escherichia coli microcultures. J. Bacteriol. 88:1151–1154. 1964.—Analysis of extensive time-lapse photomicrographic records of Escherichia coli microcultures uncovered two cases in which there occurred aberrant cell growth resembling a germination tube. Although previously observed by a number of investigators in gram-positive bacteria, the present observations appear to constitute the first time-lapse photomicrographic record concerning a gram-negative form. In the first case, with photographs taken at 15-sec intervals, the cell initially exhibited a clublike deformation, and the “germ tube” then issued from the club head. The “tube” had developed into a separate cell by the time the photographic record was concluded. In the second case, with the photographs taken at 1-min intervals over a longer period of time, the “germ tube” cell assumed a plastic dumbbell-like form after separation from its sister. It is suggested that the phenomenon results from a disturbance in cell-wall synthesis, while cytoplasmic growth continues unabated.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHAN E. C. MORPHOLOGICAL ABERRATION OF ARTHROBACTER GLOBIFORMIS CELLS DUE TO BIOTIN DEFICIENCY. J Bacteriol. 1964 Mar;87:641–651. doi: 10.1128/jb.87.3.641-651.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GILMOUR M. N., HOWELL A., Jr, BIBBY B. G. The classification of organisms termed Leptotrichia (Leptothrix) buccalis. I. Review of the literature and proposed separation into Leptotrichia buccalis Trevisan, 1879 and Bacterionema gen. nov., B. matruchotti (Mendel, 1919) comb. nov. Bacteriol Rev. 1961 Jun;25:131–141. doi: 10.1128/br.25.2.131-141.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GILMOUR M. N. The classification of organisms termed Leptotrichia (Leptothrix) buccalis. II. Reproduction of Bacterionema matruchotii. Bacteriol Rev. 1961 Jun;25:142–151. doi: 10.1128/br.25.2.142-151.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gillespie H. B., Rettger L. F. Bacterial Variation: Formation and Fate of Certain Variant Cells of Bacillus megatherium. J Bacteriol. 1939 Jul;38(1):41–61. doi: 10.1128/jb.38.1.41-61.1939. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOFFMAN H., FRANK M. E. TEMPERATURE LIMITS, GENEALOGICAL ORIGIN, DEVELOPMENTAL COURSE, AND ULTIMATE FATE OF HEAT-INDUCED FILAMENTS IN ESCHERICHIA COLI MICROCULTURES. J Bacteriol. 1963 Jun;85:1221–1234. doi: 10.1128/jb.85.6.1221-1234.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUDOH S. The growth mode of Mycobacterium under microculture. Acta Tuberc Scand. 1956;32(1):74–87. [PubMed] [Google Scholar]
- STARR M. P., KUHN D. A. On the origin of V-forms in Arthrobacter atrocyaneus. Arch Mikrobiol. 1962;42:289–298. doi: 10.1007/BF00422046. [DOI] [PubMed] [Google Scholar]