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. Author manuscript; available in PMC: 2012 Jul 19.
Published in final edited form as: Langmuir. 2011 Jun 16;27(14):8829–8840. doi: 10.1021/la201246m

Figure 10.

Figure 10

Time courses for CO (464 μM) association with human: (A) Hb (B) PEG-LEHs and (C) RBCs. The experimental data shows an average of 8–10 kinetic traces. For experiments with Hb and PEG-LEHs, both CO and Hb solutions were made in 50 mM Tris buffer (pH 7.4) in the presence of 1.5 mg/mL of sodium dithionite. For experiments with RBCs, CO and RBC, solutions were made in PBS (pH 7.4) in the presence of 1.5 mg/mL of sodium dithionite, in order to avoid hypotonic shock and lysis of RBCs. The reactions were monitored at 437.5 nm and 25°C.