Abstract
Badakhsh, Fred F. (University of Georgia, Athens), and John W. Foster. Detoxification and immunogenic properties of endotoxin-containing precipitate of Brucella abortus. J. Bacteriol. 91:494–498. 1966.—Endotoxin-containing precipitates (ECP) were prepared from Brucella abortus strain 19A by aqueous ether extraction followed by ethyl alcohol precipitation. Lysozyme was the most effective of several enzymes tried for detoxification of endotoxin present in the precipitate. Trypsin was shown to reduce mouse lethal toxicity but not rabbit dermal toxicity. Immunological studies of ECP and enzyme-treated ECP demonstrated that lysozyme did not harm the immunogenic property of ECP, whereas heat, ribonuclease, lipase, and proteolytic enzymes had an adverse effect. Serological reactivity of ECP was increased after lysozyme treatment, whereas ribonuclease reduced serological activity.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALLEN R. C. Studies on an immunogenic agent against Brucella abortus. I. Preliminary studies of an immunologically active soluble agglutinogen. Am J Vet Res. 1961 May;22:558–563. [PubMed] [Google Scholar]
- BOBO R. A., FOSTER J. W. THE EFFECT OF ENZYME TREATMENTS ON BRUCELLA ABORTUS CELL WALLS. J Gen Microbiol. 1964 Jan;34:1–8. doi: 10.1099/00221287-34-1-1. [DOI] [PubMed] [Google Scholar]
- FOSTER J. W., RIBI E. Immunological role of Brucella abortus cell walls. J Bacteriol. 1962 Aug;84:258–268. doi: 10.1128/jb.84.2.258-268.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LARSON C. L., RIBI E., MILNER K. C., LIEBERMAN J. E. A method for titrating endotoxic activity in the skin of rabbits. J Exp Med. 1960 Jan 1;111:1–20. doi: 10.1084/jem.111.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LARSON C. L. Studies on thermostable antigens extracted from Bacterium tularense and from tissues of animals dead of tularemia. J Immunol. 1951 Feb;66(2):249–259. [PubMed] [Google Scholar]
- MARKENSON J., SULITZEANU D., OLITZKI A. L. Immunizing properties of insoluble cell material derived from Brucella. Nature. 1959 Jun 13;183(4676):1693–1693. doi: 10.1038/1831693a0. [DOI] [PubMed] [Google Scholar]
- OLITZKI A. L., SULITZEANU D. Studies on the antigenic structure of Brucella suis with the aid of the agar gel precipitation technique. I. The resistance of antigens to physical, chemical and enzymatic treatments. Br J Exp Pathol. 1958 Jun;39(3):219–227. [PMC free article] [PubMed] [Google Scholar]
- RIBI E., HASKINS W. T., MILNER K. C., ANACKER R. L., RITTER D. B., GOODE G., TRAPANI R. J., LANDY M. Physicochemical changes in endotoxin associated with loss of biological potency. J Bacteriol. 1962 Oct;84:803–814. doi: 10.1128/jb.84.4.803-814.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RODE L. J., OGLESBY G., SCHUHARDT V. T. The cultivation of brucellae on chemically defined media. J Bacteriol. 1950 Nov;60(5):661–668. doi: 10.1128/jb.60.5.661-668.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMITH E. E., RUTENBURG S. H., RUTENBURG A. M., FINE J. Detoxification of endotoxin by splenic extracts. Proc Soc Exp Biol Med. 1963 Jul;113:781–784. doi: 10.3181/00379727-113-28490. [DOI] [PubMed] [Google Scholar]
- Treffers H. P. The Detoxification by Acetylation of Soluble Antigens From Shigella dysenteriae (Shiga) and E. typhosa. Science. 1946 Mar 29;103(2674):387–389. doi: 10.1126/science.103.2674.387. [DOI] [PubMed] [Google Scholar]
- WHITE P. G., WILSON J. B. Differentiation of smooth and nonsmooth colonies of Brucellae. J Bacteriol. 1951 Feb;61(2):239–240. doi: 10.1128/jb.61.2.239-240.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]