Abstract
Ensign, J. C. (University of Wisconsin, Madison), and R. S. Wolfe. Characterization of a small proteolytic enzyme which lyses bacterial cell walls. J. Bacteriol. 91:524–534. 1966.—An enzyme isolated from a myxobacter possesses both cell-wall lytic and proteolytic activity. The enzyme has been purified over 600-fold and is electrophoretically homogeneous upon cellulose acetate at several pH values and upon polyacrylamide gel columns. A single peak was obtained upon ultracentrifugation and density gradient centrifugation. Based upon Sephadex gel filtration, a molecular weight of 8,700 was determined for the enzyme. Albumin and casein were extensively degraded by the enzyme, with approximately one-third of the peptide bonds present in these proteins being hydrolyzed. The enzyme lyses cell walls by hydrolyzing peptide bonds in the glycosaminopeptide.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BROWDER H. P., ZYGMUNT W. A., YOUNG J. R., TAVORMINA P. A. LYSOSTAPHIN: ENZYMATIC MODE OF ACTION. Biochem Biophys Res Commun. 1965 Apr 23;19:383–389. doi: 10.1016/0006-291x(65)90473-0. [DOI] [PubMed] [Google Scholar]
- CUNNINGHAM L. W., Jr Molecular-kinetic properties of crystalline diisopropyl phosphoryl trypsin. J Biol Chem. 1954 Nov;211(1):13–19. [PubMed] [Google Scholar]
- DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
- ENSIGN J. C., WOLFE R. S. LYSIS OF BACTERIAL CELL WALLS BY AN ENZYME ISOLATED FROM A MYXOBACTER. J Bacteriol. 1965 Aug;90:395–402. doi: 10.1128/jb.90.2.395-402.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GHUYSEN J. M., STROMINGER J. L. STRUCTURE OF THE CELL WALL OF STAPHYLOCOCCUS AUREUS, STRAIN COPENHAGEN. I. PREPARATION OF FRAGMENTS BY ENZYMATIC HYDROLYSIS. Biochemistry. 1963 Sep-Oct;2:1110–1119. doi: 10.1021/bi00905a035. [DOI] [PubMed] [Google Scholar]
- GLAZER A. N., WELLNER D. Adsorption of proteins on 'Sephadex'. Nature. 1962 Jun 2;194:862–863. doi: 10.1038/194862a0. [DOI] [PubMed] [Google Scholar]
- HIRAMATSU A., OUCHI T. ON THE PROTEOLYTIC ENZYMES FROM THE COMMERCIAL PROTEASE PREPARATION OF STREPTOMYCES GRISEUS (PRONASE P). J Biochem. 1963 Nov;54:462–464. doi: 10.1093/oxfordjournals.jbchem.a127815. [DOI] [PubMed] [Google Scholar]
- HIRS C. H., MOORE S., STEIN W. H. Peptides obtained by tryptic hydrolysis of performic acid-oxidized ribonuclease. J Biol Chem. 1956 Apr;219(2):623–642. [PubMed] [Google Scholar]
- MARGOLIASH E. Amino acid sequence of chymotryptic peptides from horse heart cytochrome c. J Biol Chem. 1962 Jul;237:2161–2174. [PubMed] [Google Scholar]
- MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
- MONTAGUE M. D. THE ENZYME DEGRADATION OF CELL WALLS OF STREPTOCOCCUS FAECALIS. Biochim Biophys Acta. 1964 Jun 8;86:588–595. doi: 10.1016/0304-4165(64)90098-4. [DOI] [PubMed] [Google Scholar]
- ORNSTEIN L. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY. Ann N Y Acad Sci. 1964 Dec 28;121:321–349. doi: 10.1111/j.1749-6632.1964.tb14207.x. [DOI] [PubMed] [Google Scholar]
- PARK J. T., JOHNSON M. J. A submicrodetermination of glucose. J Biol Chem. 1949 Nov;181(1):149–151. [PubMed] [Google Scholar]
- PELZER H. MUCOPEPTIDHYDROLASEN IN ESCHERICHIA COLI B. I. NACHWEIS UND WIRKUNGSSPEZIFITAET. Z Naturforsch B. 1963 Nov;18:950–956. [PubMed] [Google Scholar]
- REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
- WU Y. V., SCHERAGA H. A. Studies of soybean trypsin inhibitor. I. Physicochemical properties. Biochemistry. 1962 Jul;1:698–705. doi: 10.1021/bi00910a025. [DOI] [PubMed] [Google Scholar]
- YOUNG F. E., TIPPER D. J., STROMINGER J. L. AUTOLYSIS OF CELL WALLS OF BACILLUS SUBTILIS. MECHANISM AND POSSIBLE RELATIONSHIP TO COMPETENCE. J Biol Chem. 1964 Oct;239:PC3600–PC3602. [PubMed] [Google Scholar]