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. 1966 Feb;91(2):535–545. doi: 10.1128/jb.91.2.535-545.1966

Light-Induced Lysis and Carotenogenesis in Myxococcus xanthus1

Robert P Burchard a, Martin Dworkin a,2
PMCID: PMC314892  PMID: 5935340

Abstract

Burchard, Robert P. (University of Minnesota, Minneapolis), and Martin Dworkin. Light-induced lysis and carotenogenesis in Myxococcus xanthus. J. Bacteriol. 91:535–545. 1966.—Myxococcus xanthus, grown vegetatively in the light, developed an orange carotenoid after the cells entered stationary phase of growth; pigment content increased with age. Cells grown in the dark did not develop carotenoid and could be photolysed by relatively low-intensity light only during stationary phase; rate of photolysis increased with age. Photolysis adhered to the reciprocity law, was temperature-independent and oxygen-dependent, and required the presence of nonspecific, monovalent cations; it was inhibited by one of several divalent cations. Logarithmic-phase cells were photosensitized by 100,000 × g pellet preparations of sonic-treated stationary-phase cells grown in the light and dark. A porphyrin with a Soret band at 408 mμ was isolated from photosensitive cells; logarithmic-phase cells contained about 1/16 the amount of porphyrin of stationary-phase cells. The purified material had spectral and chemical properties of protoporphyrin IX and photosensitized logarithmic-phase cells. Its spectrum was similar to the action spectrum for photolysis. We concluded that protoporphyrin IX is the natural endogenous photosensitizer. Carotenogenesis was stimulated by light in the blue-violet region of the visible spectrum and was inhibited by diphenylamine, resulting in photosensitivity of the cells. Photoprotection by carotenoid was lost in the cold. A mutant which synthesized carotenoid in the light and dark was photosensitive only after growth in diphenylamine. The ecological significance of these phenomena is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAMJI M. S., KRINSKY N. I. CAROTENOID DE-EPOXIDATIONS IN ALGAE. II. ENZYMATIC CONVERSION OF ANTHERAXANTHIN TO ZEAXANTHIN. J Biol Chem. 1965 Jan;240:467–470. [PubMed] [Google Scholar]
  2. BOGORAD L., GRANICK S. Protoporphyrin precursors produced by a Chlorella mutant. J Biol Chem. 1953 Jun;202(2):793–800. [PubMed] [Google Scholar]
  3. BROWN A. D. THE PERIPHERAL STRUCTURES OF GRAM-NEGATIVE BACTERIA.IV. THE CATION-SENSITIVE DISSOLUTION OF THE CELL MEMBRANE OF THE HALOPHILIC BACTERIUM, HALOBACTERIUM HALOBIUM. Biochim Biophys Acta. 1963 Nov 29;75:425–435. doi: 10.1016/0006-3002(63)90630-9. [DOI] [PubMed] [Google Scholar]
  4. Burchard R. P., Gordon S. A., Dworkin M. Action spectrum for the photolysis of Myxococcus xanthus. J Bacteriol. 1966 Feb;91(2):896–897. doi: 10.1128/jb.91.2.896-897.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DUNDAS I. D., LARSEN H. A STUDY ON THE KILLING BY LIGHT OF PHOTOSENSITIZED CELLS OF HALOBACTERIUM SALINARIUM. Arch Mikrobiol. 1963 Jul 18;46:19–28. doi: 10.1007/BF00406383. [DOI] [PubMed] [Google Scholar]
  6. DWORKIN M. Function of carotenoids in photosynthetic bacteria. Nature. 1959 Dec 12;184(Suppl 24):1891–1892. doi: 10.1038/1841891b0. [DOI] [PubMed] [Google Scholar]
  7. DWORKIN M., NIEDERPRUEM D. J. ELECTRON TRANSPORT SYSTEM IN VEGETATIVE CELLS AND MICROCYSTS OF MYXOCOCCUS XANTHUS. J Bacteriol. 1964 Feb;87:316–322. doi: 10.1128/jb.87.2.316-322.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DWORKIN M. Nutritional requirements for vegetative growth of Myxococcus xanthus. J Bacteriol. 1962 Aug;84:250–257. doi: 10.1128/jb.84.2.250-257.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FELDMAN L. A., LINDSTROM E. S. THE EFFECT OF CAROTENOID PIGMENTS ON PHOTOOXIDATIONS OF SOME PHOTOSYNTHETIC BACTERIA. Biochim Biophys Acta. 1964 Mar 30;79:266–272. [PubMed] [Google Scholar]
  10. GOODWIN T. W., OSMAN H. G. Studies in carotenogenesis. 9. General cultural conditions controlling carotenoid (spirilloxanthin) synthesis in the photosynthetic bacterium Rhodospirillum rubrum. Biochem J. 1953 Mar;53(4):541–546. doi: 10.1042/bj0530541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GRIFFITHS M., SISTROM W. R., COHENBAZIRE G., STANIER R. Y., CALVIN M. Function of carotenoids in photosynthesis. Nature. 1955 Dec 24;176(4495):1211–1215. doi: 10.1038/1761211a0. [DOI] [PubMed] [Google Scholar]
  12. MASON D. J., POWELSON D. The cell wall of Myxococcus xanthus. Biochim Biophys Acta. 1958 Jul;29(1):1–7. doi: 10.1016/0006-3002(58)90138-0. [DOI] [PubMed] [Google Scholar]
  13. MATHEWS M. M., SISTROM W. R. The function of the carotenoid pigments of Sarcina lutea. Arch Mikrobiol. 1960;35:139–146. doi: 10.1007/BF00425002. [DOI] [PubMed] [Google Scholar]
  14. RILLING H. C. Photoinduction of carotenoid synthesis of a Mycobacterium sp. Biochim Biophys Acta. 1962 Jul 16;60:548–556. doi: 10.1016/0006-3002(62)90873-9. [DOI] [PubMed] [Google Scholar]
  15. RUDZINSKA M. A., GRANICK S. Protoporphyrin production of Tetrahymena geleii. Proc Soc Exp Biol Med. 1953 Jul;83(3):525–526. doi: 10.3181/00379727-83-20404. [DOI] [PubMed] [Google Scholar]
  16. SISTROM W. R., GRIFFITHS M., STANIER R. Y. The biology of photosynthetic bacterium which lacks colored carotenoids. J Cell Physiol. 1956 Dec;48(3):473–515. doi: 10.1002/jcp.1030480309. [DOI] [PubMed] [Google Scholar]

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