Abstract
Cook, Thomas M. (Sterling-Winthrop Research Institute, Rensselaer, N.Y.), William H. Deitz, and William A. Goss. Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents. J. Bacteriol. 91:774–779. 1996.—Treatment of Escherichia coli 15TAU with nalidixic acid resulted in degradation of the nucleic acids of the cells, whereas protein was unaffected. Deoxyribonucleic acid (DNA) degradation appeared to be more extensive than ribonucleic acid degradation during periods of comparable bactericidal action. The onset of DNA degradation was evident prior to a measurable bactericidal effect. However, within the range of 2 to 20%, DNA degradation was accompanied by a decrease in viable cell numbers. Degradation of DNA to acid-soluble material occurred only under conditions permitting the bactericidal action of nalidixic acid. Arrest of the bactericidal action of nalidixic acid by the addition of dinitrophenol or chloramphenicol also inhibited DNA degradation. The acid-soluble products, which were excreted into the medium, have not been characterized completely, but probably were not phosphorylated.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOYCE R. P., HOWARD-FLANDERS P. GENETIC CONTROL OF DNA BREAKDOWN AND REPAIR IN E. COLI K-12 TREATED WITH MITOMYCIN C OR ULTRAVIOLET LIGHT. Z Vererbungsl. 1964 Dec 30;95:345–350. doi: 10.1007/BF01268667. [DOI] [PubMed] [Google Scholar]
- CONSTANTOPOULOS G., TCHEN T. T. ENHANCEMENT OF MITOMYCIN C-INDUCED BREAKDOWN OF DNA BY INHIBITORS OF PROTEIN SYNTHESIS. Biochim Biophys Acta. 1964 Mar 23;80:456–462. doi: 10.1016/0926-6550(64)90148-3. [DOI] [PubMed] [Google Scholar]
- Deitz W. H., Cook T. M., Goss W. A. Mechanism of action of nalidixic acid on Escherichia coli. 3. Conditions required for lethality. J Bacteriol. 1966 Feb;91(2):768–773. doi: 10.1128/jb.91.2.768-773.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOSS W. A., DEITZ W. H., COOK T. M. MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI. J Bacteriol. 1964 Oct;88:1112–1118. doi: 10.1128/jb.88.4.1112-1118.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOSS W. A., DEITZ W. H., COOK T. M. MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI.II. INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS. J Bacteriol. 1965 Apr;89:1068–1074. doi: 10.1128/jb.89.4.1068-1074.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KERSTEN H. Action of mitomycin C on nucleic acid metabolism in tumor and bacterial cells. Biochim Biophys Acta. 1962 Apr 2;55:558–560. doi: 10.1016/0006-3002(62)90994-0. [DOI] [PubMed] [Google Scholar]
- KURYLO-BOROWSKA Z. ON THE MODE OF ACTION OF EDEINE. EFFECT OF EDEINE ON THE BACTERIAL DNA. Biochim Biophys Acta. 1964 Jun 22;87:305–313. doi: 10.1016/0926-6550(64)90226-9. [DOI] [PubMed] [Google Scholar]
- REICH E., SHATKIN A. J., TATUM E. L. Bacteriocidal action of mitomycin C. Biochim Biophys Acta. 1961 Oct 14;53:132–149. doi: 10.1016/0006-3002(61)90800-9. [DOI] [PubMed] [Google Scholar]
- SHIBA S., TERAWAKI A., TAGUCHI T., KAWAMATA J. Selective inhibition of formation of deoxyribonucleic acid in Escherichia coli by mitomycin C. Nature. 1959 Apr 11;183(4667):1056–1057. doi: 10.1038/1831056a0. [DOI] [PubMed] [Google Scholar]
- SZYBALSKI W., IYER V. N. CROSSLINKING OF DNA BY ENZYMATICALLY OR CHEMICALLY ACTIVATED MITOMYCINS AND PORFIROMYCINS, BIFUNCTIONALLY "ALKYLATING" ANTIBIOTICS. Fed Proc. 1964 Sep-Oct;23:946–957. [PubMed] [Google Scholar]
- TANAKA N., YAMAGUCHI H., UMEZAWA H. Mechanism of action of phleomycin, a tumor-inhibitory antibiotic. Biochem Biophys Res Commun. 1963 Jan 31;10:171–174. doi: 10.1016/0006-291x(63)90045-7. [DOI] [PubMed] [Google Scholar]