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. 1966 Feb;91(2):784–788. doi: 10.1128/jb.91.2.784-788.1966

Role of Acetate in Sporogenesis of Bacillus cereus

H M Nakata 1
PMCID: PMC314930  PMID: 4956759

Abstract

Nakata, H. M. (Washington State University, Pullman). Role of acetate in sporogenesis of Bacillus cereus. J. Bacteriol. 91:784–788. 1966.—The distribution of radioactivity associated initially with acetate-2-C14 was followed during sporogenesis of Bacillus cereus strain T. This was accomplished by replacing cells committed to sporulation into a chemically defined sporulation medium. It was observed that 65 to 70% of the initial radioactivity was incorporated into poly-β-hydroxybutyrate, whereas 20 to 25% was found in other cellular constituents. Virtually no radioactivity was lost as C14O2 during the first 5 to 6 hr after replacement. Then, a gradual evolution of C14O2 coincident with poly-β-hydroxybutyrate degradation, was observed until about the ninth hour. By this time, the polymer was essentially depleted, and the first spore structures were observed in stained preparations. The total amount of radioactivity lost as C14O2 was 20 to 25%. The major portion of products derived from poly-β-hydroxybutyrate was incorporated into the spores. As much as 17% of the radioactivity associated with the spores was found in dipicolinic acid. More than 50% was located in spore proteins, 20 to 25% in the hot 5% trichloroacetic acid-soluble fraction, 4 to 5% in the lipid fraction, and 15 to 20% in the cold 5% trichloroacetic acid-soluble fraction. These data, accounting for 70 to 75% of the initial radioactivity, confirmed the hypothesis that the major role of acetate, and subsequently of poly-β-hydroxybutyrate, in sporulation of B. cereus T is to provide carbon precursors and energy for sporogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CANFIELD R. E., SZULMAJSTER J. TIME OF SYNTHESIS OF DEOXYRIBONUCLEIC ACID AND PROTEIN IN SPORES OF B. SUBTILIS. Nature. 1964 Aug 8;203:596–598. doi: 10.1038/203596a0. [DOI] [PubMed] [Google Scholar]
  2. FOSTER J. W., PERRY J. J. Intracellular events occurring during endotrophic sporulation in Bacillus mycoides. J Bacteriol. 1954 Mar;67(3):295–302. doi: 10.1128/jb.67.3.295-302.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HANSON R. S., SRINIVASAN V. R., HALVORSON H. O. Biochemistry of sporulation. I. Metabolism of acetate by vegetative and sporulating cells. J Bacteriol. 1963 Feb;85:451–460. doi: 10.1128/jb.85.2.451-460.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. JANSSEN F. W., LUND A. J., ANDERSON L. E. Colorimetric assay for dipicolinic acid in bacterial spores. Science. 1958 Jan 3;127(3288):26–27. doi: 10.1126/science.127.3288.26. [DOI] [PubMed] [Google Scholar]
  5. LAW J. H., SLEPECKY R. A. Assay of poly-beta-hydroxybutyric acid. J Bacteriol. 1961 Jul;82:33–36. doi: 10.1128/jb.82.1.33-36.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MARTIN H. H., FOSTER J. W. Biosynthesis of dipicolinic acid in Bacillus megaterium. J Bacteriol. 1958 Aug;76(2):167–178. doi: 10.1128/jb.76.2.167-178.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MILLET J., AUBERT J. P. [The metabolism of glutamic acid in the course of sporulation in Bacillus megaterium]. Ann Inst Pasteur (Paris) 1960 Feb;98:282–290. [PubMed] [Google Scholar]
  8. NAKATA H. M. EFFECT OF PH ON INTERMEDIATES PRODUCED DURING GROWTH AND SPORULATION OF BACILLUS CEREUS. J Bacteriol. 1963 Sep;86:577–581. doi: 10.1128/jb.86.3.577-581.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. NAKATA H. M., HALVORSON H. O. Biochemical changes occurring during growth and sporulation of Bacillus cereus. J Bacteriol. 1960 Dec;80:801–810. doi: 10.1128/jb.80.6.801-810.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NAKATA H. M. ORGANIC NUTRIENTS REQUIRED FOR GROWTH AND SPORULATION OF BACILLUS CEREUS. J Bacteriol. 1964 Nov;88:1522–1524. doi: 10.1128/jb.88.5.1522-1524.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PARK J. T., HANCOCK R. A fractionation procedure for studies of the synthesis of cell-wall mucopeptide and of other polymers in cells of Staphylococcus aureus. J Gen Microbiol. 1960 Feb;22:249–258. doi: 10.1099/00221287-22-1-249. [DOI] [PubMed] [Google Scholar]
  12. POWELL J. F., STRANGE R. E. Synthesis of dipicolinic acid from 2,6-diketopimelic acid. Nature. 1959 Sep 19;184:878–880. doi: 10.1038/184878a0. [DOI] [PubMed] [Google Scholar]
  13. TANENBAUM S. W., KANEKO K. BIOSYNTHESIS OF DIPICOLINIC ACID AND OF LYSINE IN PENICILLIUM CITREO-VIRIDE. Biochemistry. 1964 Sep;3:1314–1322. doi: 10.1021/bi00897a022. [DOI] [PubMed] [Google Scholar]

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