Abstract
Margaretten, William (College of Physicians and Surgeons of Columbia University, New York, N.Y.), Councilman Morgan, Herbert S. Rosenkranz, and Harry M. Rose. Effect of hydroxyurea on virus development. I. Electron microscopic study of the effect on the development of bacteriophage T4. J. Bacteriol. 91:823–833. 1966.—Double fixation in gluteraldehyde and osmium tetroxide and the application of lead staining revealed details of viral structure not previously observed in thin sections. Bacteriophage presumed to have injected its deoxyribonucleic acid (DNA) exhibited a dense, hollow, disc-shaped core. Within the cytoplasm of infected bacteria, the peripheral membrane of the viral heads was clearly visible. Aberrant forms containing the hollow core and believed to be devoid of DNA were encountered in studies of the normal course of development. Hydroxyurea, which is believed to interfere with the production of infective bacteriophage by inhibiting DNA synthesis, resulted in the appearance of viral particles with the hollow disc or with bizarre, distorted cores. However, a significant number of viral heads looked entirely normal and presumably contained a full complement of DNA. Hypotheses are presented to explain these observations.
Full text
PDF










Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- COTA-ROBLES E. H., COFFMAN M. D. FINE STRUCTURE OF THE BACTERIOPHAGE ATTACHMENT PROCESS. J Bacteriol. 1963 Aug;86:266–273. doi: 10.1128/jb.86.2.266-273.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CUMMINGS D. J., WANKO T. AN ELECTRON MICROSCOPIC STUDY OF T2 BACTERIOPHAGE IN THIN SECTION. J Mol Biol. 1963 Dec;7:658–661. doi: 10.1016/s0022-2836(63)80113-8. [DOI] [PubMed] [Google Scholar]
- DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOERMANN A. H. The intracellular growth of bacteriophages. I. Liberation of intracellular bacteriophage T4 by premature lysis with another phage or with cyanide. J Gen Physiol. 1952 Mar;35(4):645–656. doi: 10.1085/jgp.35.4.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HATTMAN S., FUKASAWA T. HOST-INDUCED MODIFICATION OF T-EVEN PHAGES DUE TO DEFECTIVE GLUCOSYLATION OF THEIR DNA. Proc Natl Acad Sci U S A. 1963 Aug;50:297–300. doi: 10.1073/pnas.50.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELLENBERGER E., SECHAUD J., RYTER A. Electron microscopical studies of phage multiplication. IV. The establishment of the DNA pool of vegetative phage and the maturation of phage particles. Virology. 1959 Aug;8:478–498. doi: 10.1016/0042-6822(59)90050-9. [DOI] [PubMed] [Google Scholar]
- KELLENBERGER E. Vegetative bacteriophage and the maturation of the virus particles. Adv Virus Res. 1961;8:1–61. doi: 10.1016/s0065-3527(08)60682-x. [DOI] [PubMed] [Google Scholar]
- LURIA S. E. Bacteriophage genes and bacterial functions. Science. 1962 May 25;136(3517):685–692. doi: 10.1126/science.136.3517.685. [DOI] [PubMed] [Google Scholar]
- LURIA S. E., HUMAN M. L. Chromatin staining of bacteria during bacteriophage infection. J Bacteriol. 1950 Apr;59(4):551–560. doi: 10.1128/jb.59.4.551-560.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAALØE O., BIRCH-ANDERSEN A., SJOSTRAND F. S. Electron micrographs of sections of E. coli cells infected with the bacteriophage T4. Biochim Biophys Acta. 1954 Sep;15(1):12–19. doi: 10.1016/0006-3002(54)90087-6. [DOI] [PubMed] [Google Scholar]
- MURRAY R. G. E., GILLEN D. H., HEAGY F. C. Cytological changes in Escherichia coli produced by infection with phage T2. J Bacteriol. 1950 May;59(5):603–615. doi: 10.1128/jb.59.5.603-615.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSENKRANZ H. S., LEVY J. A. HYDROXYUREA: A SPECIFIC INHIBITOR OF DEOXYRIBONUCLEIC ACID SYNTHESIS. Biochim Biophys Acta. 1965 Jan 11;95:181–183. doi: 10.1016/0005-2787(65)90225-x. [DOI] [PubMed] [Google Scholar]
- SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHEDLOVSKY A., BRENNER S. A CHEMICAL BASIS FOR THE HOST-INDUCED MODIFICATION OF T-EVEN BACTERIOPHAGES. Proc Natl Acad Sci U S A. 1963 Aug;50:300–305. doi: 10.1073/pnas.50.2.300. [DOI] [PMC free article] [PubMed] [Google Scholar]