Abstract
Hyperimmune sera against spherules and against arthrospores of Coccidioides immitis were prepared by inoculation of rabbits. The antibody content of these sera was studied by the agar gel diffusion method. It was observed that antispherule pooled sera formed multiple precipitin bands with extracts of spherules and of arthrospores. The antiarthrospore pooled serum, however, failed to precipitate with the spherule extract, and formed a single band in the presence of an arthrospore solution. When the spherule and the arthrospore extracts were tested with a variety of different antisera, it was observed that the spherule preparation formed bands only in combination with anti-purified spherule pooled serum, whereas the arthrospore extract precipitated with anti-purified spherule, antiarthrospore, and anti-Histoplasma capsulatum pooled sera. It was also observed that a spherule culture supernatant solution formed five precipitin bands in combination with anti-spherule pooled sera, formed one band with pooled antiserum from rabbits with coccidioidomycosis, and did not precipitate in the presence of antiarthrospore pooled serum. Coccidioidin, however, formed two bands in the presence of any of these antisera. It was therefore concluded that extracts from the spherule phase of C. immitis differed from solutions obtained from the arthrospore and mycelial phases.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CAMPBELL C. C., BINKLEY G. E. Serologic diagnosis with respect to histoplasmosis, coccidioidomycosis, and blastomycosis and the problem of cross reactions. J Lab Clin Med. 1953 Dec;42(6):896–906. [PubMed] [Google Scholar]
- CAMPBELL C. C. The accuracy of serologic methods in diagnosis. Ann N Y Acad Sci. 1960 Aug 27;89:163–177. doi: 10.1111/j.1749-6632.1960.tb20139.x. [DOI] [PubMed] [Google Scholar]
- CONVERSE J. L. Effect of surface active agents on endosporulation of Coccidioides immitis in a chemically defined medium. J Bacteriol. 1957 Jul;74(1):106–107. doi: 10.1128/jb.74.1.106-107.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HILL G. B., CAMPBELL C. C. A further evaluation of histoplasmin and yeast phase antigen of Histoplasma capsulatum in the complement fixation test. J Lab Clin Med. 1956 Aug;48(2):255–263. [PubMed] [Google Scholar]
- LEVINE H. B., COBB J. M., SMITH C. E. Immunity to coccidioi-domycosis induced in mice by purified spherule, arthrospore, and mycelial vaccines. Trans N Y Acad Sci. 1960 Apr;22:436–449. doi: 10.1111/j.2164-0947.1960.tb00711.x. [DOI] [PubMed] [Google Scholar]
- LEVINE H. B., MILLER R. L., SMITH C. E. Influence of vaccination on respiratory coccidiodial disease in cynomolgous monkeys. J Immunol. 1962 Aug;89:242–251. [PubMed] [Google Scholar]
- LEVINE H. B. Purification of the spherule-endospore phase of Coccidioides immitis. Sabouraudia. 1961 Jun;1:112–115. doi: 10.1080/00362176285190231. [DOI] [PubMed] [Google Scholar]
- MARTIN D. S. Serologic studies on North American blastomycosis; studies with soluble antigens from untreated and sonic-treated yeast-phase cells of Blastomyces dermatitidis. J Immunol. 1953 Oct;71(4):192–201. [PubMed] [Google Scholar]
- SCHUBERT J. H., AJELLO L., COOPER J. S., RUNYON L. C. Evaluation of histoplasmin and yeast phase antigens derived from a single strain of Histoplasma capsulatum in the complement fixation test. J Bacteriol. 1955 May;69(5):558–562. doi: 10.1128/jb.69.5.558-562.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMITH C. E., SAITO M. T., BEARD R. R., KEPP R. M., CLARK R. W., EDDIE B. U. Serological tests in the diagnosis and prognosis of coccidioidomycosis. Am J Hyg. 1950 Jul;52(1):1–21. doi: 10.1093/oxfordjournals.aje.a119404. [DOI] [PubMed] [Google Scholar]
- VOGEL R. A., FETTER B. F., CONANT N. F., LOWE E. P. Preliminary studies on artificial active immunization of guinea pigs against respiratory challenge with Coccidioides immitis. Am Rev Tuberc. 1954 Sep;70(3):498–503. doi: 10.1164/art.1954.70.3.498. [DOI] [PubMed] [Google Scholar]