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. 1967 Jan;93(1):21–26. doi: 10.1128/jb.93.1.21-26.1967

Some Properties of Heat-Resistant and Heat-Sensitive Strains of Clostridium perfringens I. Heat Resistance and Toxigenicity1

Karl F Weiss a, Dorothy H Strong a
PMCID: PMC314962  PMID: 4289809

Abstract

Heat resistance at 100 C (D-values), sporulating ratios, toxigenicity for mice, and lecithinase activity (as micrograms per milliliter of enzyme, ascertained by the lecithovitellin reaction) were determined for four strains of Clostridium perfringens. A definite inverse relationship between thermal resistance and toxigenicity was found. The D-values ranged from 17.6 for the most heat-resistant strain to 0.3 for the strain possessing the least heat resistance, with corresponding lecithinase activities from 25 to 133 μg/ml of enzyme. The sporulating ratios did not differ greatly between the strains. The heat stability of the toxin was greater at 100 C than at 75 C. There was a noticeable difference between the heat stabilities of the toxin in the culture fluids of the heat-sensitive and heat-resistant strains at pH 7.0 when the toxic filtrates were held at 100 C. At a holding temperature of 75 C, a similar but lesser difference was observed at pH 5.5. Heat resistance and lecithinase activity did not change when a substrain of the least heat-resistant parent strain was obtained through heat selection by a single transfer, or when the most heat-resistant strain was transferred serially 12 times.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANGELOTTI R., HALL H. E., FOTER M. J., LEWIS K. H. Quantitation of Clostridium perfringens in foods. Appl Microbiol. 1962 May;10:193–199. doi: 10.1128/am.10.3.193-199.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ELLNER P. D. Fate of partially purified C-14-labeled toxin of Clostridium perfringens. J Bacteriol. 1961 Aug;82:275–283. doi: 10.1128/jb.82.2.275-283.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GOUDIE J. G. The nature of a neutralising substance for Clostridium welchii alpha-toxin in faeces. J Pathol Bacteriol. 1959 Jul;78:17–28. doi: 10.1002/path.1700780104. [DOI] [PubMed] [Google Scholar]
  4. HALL H. E., ANGELOTTI R., LEWIS K. H., FOTER M. J. CHARACTERISTICS OF CLOSTRIDIUM PERFRINGENS STRAINS ASSOCIATED WITH FOOD AND FOOD-BORNE DISEASE. J Bacteriol. 1963 May;85:1094–1103. doi: 10.1128/jb.85.5.1094-1103.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HOBBS B. C., SMITH M. E., OAKLEY C. L., WARRACK G. H., CRUICKSHANK J. C. Clostridium welchii food poisoning. J Hyg (Lond) 1953 Mar;51(1):75–101. doi: 10.1017/s0022172400015515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JAYKO L. G., LICHSTEIN H. C. Nutritional factors concerned with growth and lecithinase production by Clostridium perfringens. J Infect Dis. 1959 Mar-Apr;104(2):142–151. doi: 10.1093/infdis/104.2.142. [DOI] [PubMed] [Google Scholar]
  7. KUSHNER D. J. An evaluation of the egg-yolk reaction as a test for lecithinase activity. J Bacteriol. 1957 Mar;73(3):297–302. doi: 10.1128/jb.73.3.297-302.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Macfarlane M. G., Knight B. C. The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins. Biochem J. 1941 Sep;35(8-9):884–902. doi: 10.1042/bj0350884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McNICOL M., McKILLOP E. J. Foodpoisoning caused by Clostridium welchii in cold chicken. Lancet. 1958 Apr 12;1(7024):787–789. doi: 10.1016/s0140-6736(58)91595-2. [DOI] [PubMed] [Google Scholar]
  10. NISHIDA S., NAKAGAWARA G. ISOLATION OF TOXIGENIC STRAINS OF CLOSTRIDIUM NOVYI FROM SOIL. J Bacteriol. 1964 Dec;88:1636–1640. doi: 10.1128/jb.88.6.1636-1640.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NISHIDA S., NAKAGAWARA G. RELATIONSHIP BETWEEN TOXIGENICITY AND SPORULATING POTENCY OF CLOSTRIDIUM NOVYI. J Bacteriol. 1965 Apr;89:993–995. doi: 10.1128/jb.89.4.993-995.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NISIDA S., TAMAI K., YAMAGISHI T. TAXONOMY OF CLOSTRIDIUM BIFERMENTANS AND CLOSTRIDIUM SORDELLII. I. THEIR TOXIGENICITY, UREASE ACTIVITY, AND SPORULATING POTENCY. J Bacteriol. 1964 Dec;88:1641–1646. doi: 10.1128/jb.88.6.1641-1646.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NYGREN B. Phospholipase C-producing bacteria and food poisoning. An experimental study on Clostridium perfringens and Bacillus cereus. Acta Pathol Microbiol Scand Suppl. 1962;Suppl 160:1–88. [PubMed] [Google Scholar]
  14. SMITH L. D., GARDNER M. V. The anomalous heat inactivation of Clostridium perfringens lecithinase. Arch Biochem. 1950 Jan;25(1):54–60. [PubMed] [Google Scholar]
  15. TAMAI K., NISHIDA S. TAXONOMY OF CLOSTRIDIUM BIFERMENTANS AND CLOSTRIDIUM SORDELLII. II. TOXIGENIC AND SPORULATING POTENCIES IN SUBSTRAINS OF A CLOSTRIDIUM SORDELLII STRAIN. J Bacteriol. 1964 Dec;88:1647–1651. doi: 10.1128/jb.88.6.1647-1651.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Van Heyningen W. E. The biochemistry of the gas gangrene toxins: Estimation of the alpha toxin of Cl. welchii, type A. Biochem J. 1941 Nov;35(10-11):1246–1256. doi: 10.1042/bj0351246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weiss K. F., Strong D. H., Groom R. A. Mice and monkeys as assay animals for Clostridium perfringens food poisoning. Appl Microbiol. 1966 Jul;14(4):479–485. doi: 10.1128/am.14.4.479-485.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. YAMAGISHI T., ISHIDA S., NISHIDA S. ISOLATION OF TOXIGENIC STRAINS OF CLOSTRIDIUM PERFRINGENS FROM SOIL. J Bacteriol. 1964 Sep;88:646–652. doi: 10.1128/jb.88.3.646-652.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]

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