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. 1967 Jan;93(1):36–39. doi: 10.1128/jb.93.1.36-39.1967

Metabolic Changes in Nicotinamide Adenine Dinucleotide in Response to Anthrax Toxin

Irving Gray 1, Luis J Archer 1
PMCID: PMC314964  PMID: 4289811

Abstract

Bacillus anthracis produces a toxin both in vitro and in vivo which, when injected intravenously into rats, brings about the death of the animals accompanied by gross pulmonary edema. Lung tissue removed prior to death showed, in vitro, a 30% reduction in overall oxidative metabolism (Qo2), whereas the nicotinamide adenine dinucleotide (NAD)-independent succinic dehydrogenase remained unaffected. The NAD concentration in the lungs of injected animals was reduced by 50%. Upon addition of NAD, the Qo2 of lung tissue from injected animals rose to control values. At 45 min after toxin injection, the serum lactate concentration began to rise, showing about a 3.5-fold increase over controls after 75 min. No changes occurred in the pyruvate concentration. These changes may be explained by increased use of the pyruvate for glycolytic energy production with further loss of NAD. Additional experiments with liver, spleen, kidney, and brain tissues showed that the toxin-induced reduction of Qo2 is an effect specific for lung tissue. Brain tissue showed a significant increase in oxidative metabolism upon the addition of the toxin, whereas the other tissues remained unaffected. It is suggested that a principal effect of the toxin is to inhibit, in lung tissue, the regeneration of NAD in the respiratory chain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEALL F. A., TAYLOR M. J., THORNE C. B. Rapid lethal effect in rats of a third component found upon fractionating the toxin of Bacillus anthracis. J Bacteriol. 1962 Jun;83:1274–1280. doi: 10.1128/jb.83.6.1274-1280.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beall F. A., Dalldorf F. G. The pathogenesis of the lethal effect of anthrax toxin in the rat. J Infect Dis. 1966 Jun;116(3):377–389. doi: 10.1093/infdis/116.3.377. [DOI] [PubMed] [Google Scholar]
  3. Gray I., Archer L. J. Effect of anthrax toxin on oxidative metabolism of lung tissues. Am J Physiol. 1966 Jun;210(6):1313–1315. doi: 10.1152/ajplegacy.1966.210.6.1313. [DOI] [PubMed] [Google Scholar]
  4. HAINES B. W., KLEIN F., LINCOLN R. E. QUANTITATIVE ASSAY FOR CRUDE ANTHRAX TOXINS. J Bacteriol. 1965 Jan;89:74–83. doi: 10.1128/jb.89.1.74-83.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HARRIS-SMITH P. W., SMITH H., KEPPIE J. Production in vitro of the toxin of Bacillus anthracis previously recognized in vivo. J Gen Microbiol. 1958 Aug;19(1):91–103. doi: 10.1099/00221287-19-1-91. [DOI] [PubMed] [Google Scholar]
  6. Klein F., Walker J. S., Fitzpatrick D. F., Lincoln R. E., Mahlandt B. G., Jones W. I., Jr, Dobbs J. P., Hendrix K. J. Pathophysiology of anthrax. J Infect Dis. 1966 Apr;116(2):123–138. doi: 10.1093/infdis/116.2.123. [DOI] [PubMed] [Google Scholar]
  7. NORDBERG B. K., SCHMITERLOW C. G., HANSEN H. J. Pathophysiological investigations into the terminal course of experimental anthrax in the rabbit. Acta Pathol Microbiol Scand. 1961;53:295–318. doi: 10.1111/j.1699-0463.1961.tb00412.x. [DOI] [PubMed] [Google Scholar]
  8. SLEIN M. W., LOGAN G. F., Jr Mechanism of action of the toxin of Bacillus anthracis. I. Effect in vivo on some blood serum components. J Bacteriol. 1960 Jul;80:77–85. doi: 10.1128/jb.80.1.77-85.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SMITH H., KEPPIE J., STANLEY J. L. The chemical basis of the virulence of Bacillus anthracis. V. The specific toxin produced by B. Anthracis in vivo. Br J Exp Pathol. 1955 Oct;36(5):460–472. [PMC free article] [PubMed] [Google Scholar]
  10. STANLEY J. L., SMITH H. Purification of factor I and recognition of a third factor of the anthrax toxin. J Gen Microbiol. 1961 Sep;26:49–63. doi: 10.1099/00221287-26-1-49. [DOI] [PubMed] [Google Scholar]
  11. STANLEY J. L., SMITH H. The three factors of anthrax toxin: their immunogenicity and lack of demonstrable enzymic activity. J Gen Microbiol. 1963 May;31:329–337. doi: 10.1099/00221287-31-2-329. [DOI] [PubMed] [Google Scholar]
  12. THORNE C. B., MOLNAR D. M., STRANGE R. E. Production of toxin in vitro by Bacillus anthracis and its spearation into two components. J Bacteriol. 1960 Mar;79:450–455. doi: 10.1128/jb.79.3.450-455.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]

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