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. 1967 Jan;93(1):115–121. doi: 10.1128/jb.93.1.115-121.1967

Heterogeneity of the Stability of Messenger Ribonucleic Acid in Salmonella typhimurium1

J K McClatchy a,2, H V Rickenberg a,2
PMCID: PMC314976  PMID: 5335888

Abstract

Cells of Salmonella typhimurium strain SL 282, deflagellated by mechanical shear, regenerated their flagella in the absence of tryptophan, an amino acid required for growth but not found in flagellin. Ribonucleic acid (RNA) synthesis was severely inhibited by tryptophan starvation. These findings suggested that the messenger RNA (mRNA) for flagellin might be stable. Actinomycin D was used to inhibit RNA synthesis in ethylenediaminetetraacetate-treated bacteria. The introduction of an Flac episome into strain SL 282 permitted the simultaneous study of the synthesis of flagellin, β-galactosidase, and total protein. In the actinomycin-treated bacteria protein and β-galactosidase syntheses were inhibited by 90%, whereas flagellin synthesis was unaffected. We conclude that the mRNA for flagellin synthesis is stable and that species of mRNA vary with respect to metabolic stability in S. typhimurium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAM D., KOFFLER H. IN VITRO FORMATION OF FLAGELLA-LIKE FILAMENTS AND OTHER STRUCTURES FROM FLAGELLIN. J Mol Biol. 1964 Jul;9:168–185. doi: 10.1016/s0022-2836(64)80098-x. [DOI] [PubMed] [Google Scholar]
  2. ARONSON A. I., ROSASDELVALLE M. RNA AND PROTEIN SYNTHESIS REQUIRED FOR BACTERIAL SPORE FORMATION. Biochim Biophys Acta. 1964 Jun 22;87:267–276. doi: 10.1016/0926-6550(64)90222-1. [DOI] [PubMed] [Google Scholar]
  3. Ezekiel D. H. Intracellular charging of soluble ribonucleic acid in Escherichia coli subjected to isoleucine starvation and chloramphenicol treatment. Biochem Biophys Res Commun. 1964;14:64–68. doi: 10.1016/0006-291x(63)90212-2. [DOI] [PubMed] [Google Scholar]
  4. HARTWELL L. H., MAGASANIK B. THE MECHANISM OF HISTIDASE INDUCTION AND FORMATION IN BACILLUS SUBTILIS. J Mol Biol. 1964 Oct;10:105–119. doi: 10.1016/s0022-2836(64)80031-0. [DOI] [PubMed] [Google Scholar]
  5. KEPES A. KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963 Oct 15;76:293–309. [PubMed] [Google Scholar]
  6. KERRIDGE D. FLAGELLAR SYNTHESIS IN SALMONELLA TYPHIMURIUM: THE INCORPORATION OF ISOTOPICALLY-LABELLED AMINO ACIDS INTO FLAGELLIN. J Gen Microbiol. 1963 Oct;33:63–76. doi: 10.1099/00221287-33-1-63. [DOI] [PubMed] [Google Scholar]
  7. KERRIDGE D. Synthesis of flagella by amino acid-requiring mutants of Salmonella typhimurium. J Gen Microbiol. 1959 Aug;21:168–179. doi: 10.1099/00221287-21-1-168. [DOI] [PubMed] [Google Scholar]
  8. KURLAND C. G., MAALOE O. Regulation of ribosomal and transfer RNA synthesis. J Mol Biol. 1962 Mar;4:193–210. doi: 10.1016/s0022-2836(62)80051-5. [DOI] [PubMed] [Google Scholar]
  9. Kerridge D. Flagellar synthesis in Salmonella typhimurium: factors affecting the formation of the flagellar epsilon-N-methyllysine. J Gen Microbiol. 1966 Jan;42(1):71–82. doi: 10.1099/00221287-42-1-71. [DOI] [PubMed] [Google Scholar]
  10. LEIVE L. A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTA. Proc Natl Acad Sci U S A. 1965 Apr;53:745–750. doi: 10.1073/pnas.53.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LEVINTHAL C., KEYNAN A., HIGA A. Messenger RNA turnover and protein synthesis in B. subtilis inhibited by actinomycin D. Proc Natl Acad Sci U S A. 1962 Sep 15;48:1631–1638. doi: 10.1073/pnas.48.9.1631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOVELESS A., HOWARTH S. Mutation of bacteria at high levels of survival by ethyl methane sulphonate. Nature. 1959 Dec 5;184:1780–1782. doi: 10.1038/1841780a0. [DOI] [PubMed] [Google Scholar]
  13. MONOD J., COHEN-BAZIRE G., COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. doi: 10.1016/0006-3002(51)90072-8. [DOI] [PubMed] [Google Scholar]
  14. MUELLER-HILL B., RICKENBERG H. V., WALLENFELS K. SPECIFICITY OF THE INDUCTION OF THE ENZYMES OF THE LAC OPERON IN ESCHERICHIA COLI. J Mol Biol. 1964 Nov;10:303–318. doi: 10.1016/s0022-2836(64)80049-8. [DOI] [PubMed] [Google Scholar]
  15. Martinez R. J., Gordee E. Z. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in spirillum serpens and bacillus subtilis. J Bacteriol. 1966 Feb;91(2):870–875. doi: 10.1128/jb.91.2.870-875.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Martinez R. J. The formation of bacterial flagella. II. The relative stability of messenger RNA for flagellin biosynthesis. J Mol Biol. 1966 May;17(1):10–17. doi: 10.1016/s0022-2836(66)80090-6. [DOI] [PubMed] [Google Scholar]
  17. NAKADA D., MAGASANIK B. THE ROLES OF INDUCER AND CATABOLITE REPRESSOR IN THE SYNTHESIS OF BETA-GALACTOSIDASE BY ESCHERICHIA COLI. J Mol Biol. 1964 Jan;8:105–127. doi: 10.1016/s0022-2836(64)80153-4. [DOI] [PubMed] [Google Scholar]
  18. POLLOCK M. R. THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D ON THE BIOSYNTHESIS OF ENZYMES IN BACILLUS SUBTILIS AND BACILLUS CEREUS. Biochim Biophys Acta. 1963 Sep 17;76:80–93. [PubMed] [Google Scholar]
  19. SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]
  20. SEKIGUCHI M., COHEN S. S. THE SYNTHESIS OF MESSENGER RNA WITHOUT PROTEIN SYNTHESIS. II. SYNTHESIS OF PHAGE-INDUCED RNA AND SEQUENTIAL ENZYME PRODUCTION. J Mol Biol. 1964 May;8:638–659. doi: 10.1016/s0022-2836(64)80114-5. [DOI] [PubMed] [Google Scholar]
  21. STENT G. S., BRENNER S. A genetic locus for the regulation of ribonucleic acid synthesis. Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005–2014. doi: 10.1073/pnas.47.12.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]

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